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Catalog
E039693
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species
Human
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman TPSAB1 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyBrain Disorders & Neuroscience; Biogenic Amines & Neurology; Research Assays; Assay Categories
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Product Description
specificalIntended Uses||Enzyme Immunoassay for the determination of Tryptophan In urine, serum and plasma samples. For research use only, not for use in diagnostic procedures.
Principle of the Assay: After extraction and denvatizatlon Tryptophan is determined by ELISA. The competitive ELISA uses the microtlter plate format. The antigen IS bound to the solid phase of the microtiter plate. The acylated standards, controls and samples and the solid phase bound analyte compete for a fixed number of antibody binding sites. When the system is in equilibrium, free antigen and free antigen-antibody complexes are removed by washing. The antibody bound to the solid phase is detected by an anti-rabbit IgG-peroxidase conjugate using TMB as a substrate. The reaction is monitored at 450 nm. Determination of unknown samples is achieved by comparing their absorbance with a standard curve prepared with known standards.
Background/Introduction: L-Tryptophan is one of the essential amino acids for the human metnbolism and must be part of its diet. In humans it serves as precursor for the synthesis of the neurotransmitters serotonin and tryptamine as well as for the synthesis of nicotinic acid and the epiphyseal hormone melatonin. Tryptophan is catabolized to kynurenine through the enzyme IDO (lndoleamlne-2,3-dloxygenase). Increased IDO activity IS an expression of neuro-endocnne-Immunologlcal dysregulation, which is often associated with depressive symptoms such as bipolar disorder (manic depression). In addition Tryptophan and its metabolites regulate neurobehavioral effects such as appetite, sleeping-waking-rhythm and pain perception.
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