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Alternative name
Tryptase-2 ELISA KIT;
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Catalog
E039673
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species
Human
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GeneTPS
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TPS. No significant cross-reactivity or interference between TPS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TPS and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman TPS ELISA Kit allows for the in vitro quantitative determination of TPS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology
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Product Description
specificalIntended Uses: This TPS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human TPS. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||TPS ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TPS. Standards or samples are then added to the microtiter plate wells and TPS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TPS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TPS are added to each well to "sandwich" the TPS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TPS and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TPS concentration in each sample is interpolated from this standard curve.
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