Human ABCB5 ELISA Kit

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  • Alternative name

    Human ABCB5 P-gp ELISA Kit;Human P-glycoprotein ABCB5 ELISA Kit;Human ABCB5alpha ELISA Kit;Human ABCB5beta ELISA Kit;Human EST422562 ELISA Kit;Human ATP binding cassette subfamily B member 5 ELISA Kit;Human ATP-binding cassette sub-family B member 5 ELISA Kit;Human ATP-binding cassette protein ELISA Kit;Human ATP-binding cassette, sub-family B (MDR/TAP), member 5 ELISA Kit;

  • Catalog
    E003965
  • species
    Human
  • GeneABCB5
  • Standard CurveHuman ABCB5 ELISA Kit
  • Other Species Mouse Abcb5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ABCB5. No significant cross-reactivity or interference between ABCB5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ABCB5 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ABCB5 ELISA Kit allows for the in vitro quantitative determination of ABCB5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This ABCB5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ABCB5. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ABCB5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ABCB5 antibody and an ABCB5-HRP conjugate. The assay sample and buffer are incubated together with ABCB5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ABCB5 concentration since ABCB5 from samples and ABCB5-HRP conjugate compete for the anti-ABCB5 antibody binding site. Since the number of sites is limited, as more sites are occupied by ABCB5 from the sample, fewer sites are left to bind ABCB5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ABCB5 concentration in each sample is interpolated from this standard curve.
  • Human ATP-binding cassette sub-family B member 5 Protein information
  • Uniprot ID ABCB5_HUMAN
  • Uniprot AC Q2M3I5; Q5I5Q7; Q5I5Q8; Q6KG50; Q6XFQ5; Q8IXA1;
  • UniGene Hs.404102;
  • GeneID 340273
  • KEGG hsa:340273;
  • Human ATP-binding cassette sub-family B member 5 Protein SEQUENCE
  • SEQUENCE 1257 AA; 138641 MW; A4521B83E0D234CF CRC64;

    MENSERAEEM QENYQRNGTA EEQPKLRKEA VGSIEIFRFA DGLDITLMIL

    GILASLVNGA CLPLMPLVLG EMSDNLISGC LVQTNTTNYQ NCTQSQEKLN

    EDMTLLTLYY VGIGVAALIF GYIQISLWII TAARQTKRIR KQFFHSVLAQ

    DIGWFDSCDI GELNTRMTDD IDKISDGIGD KIALLFQNMS TFSIGLAVGL

    VKGWKLTLVT LSTSPLIMAS AAACSRMVIS LTSKELSAYS KAGAVAEEVL

    SSIRTVIAFR AQEKELQRYT QNLKDAKDFG IKRTIASKVS LGAVYFFMNG

    TYGLAFWYGT SLILNGEPGY TIGTVLAVFF SVIHSSYCIG AAVPHFETFA

    IARGAAFHIF QVIDKKPSID NFSTAGYKPE SIEGTVEFKN VSFNYPSRPS

    IKILKGLNLR IKSGETVALV GLNGSGKSTV VQLLQRLYDP DDGFIMVDEN

    DIRALNVRHY RDHIGVVSQE PVLFGTTISN NIKYGRDDVT DEEMERAARE

    ANAYDFIMEF PNKFNTLVGE KGAQMSGGQK QRIAIARALV RNPKILILDE

    ATSALDSESK SAVQAALEKA SKGRTTIVVA HRLSTIRSAD LIVTLKDGML

    AEKGAHAELM AKRGLYYSLV MSQDIKKADE QMESMTYSTE RKTNSLPLHS

    VKSIKSDFID KAEESTQSKE ISLPEVSLLK ILKLNKPEWP FVVLGTLASV

    LNGTVHPVFS IIFAKIITMF GNNDKTTLKH DAEIYSMIFV ILGVICFVSY

    FMQGLFYGRA GEILTMRLRH LAFKAMLYQD IAWFDEKENS TGGLTTILAI

    DIAQIQGATG SRIGVLTQNA TNMGLSVIIS FIYGWEMTFL ILSIAPVLAV

    TGMIETAAMT GFANKDKQEL KHAGKIATEA LENIRTIVSL TREKAFEQMY

    EEMLQTQHRN TSKKAQIIGS CYAFSHAFIY FAYAAGFRFG AYLIQAGRMT

    PEGMFIVFTA IAYGAMAIGE TLVLAPEYSK AKSGAAHLFA LLEKKPNIDS

    RSQEGKKPDT CEGNLEFREV SFFYPCRPDV FILRGLSLSI ERGKTVAFVG

    SSGCGKSTSV QLLQRLYDPV QGQVLFDGVD AKELNVQWLR SQIAIVPQEP

    VLFNCSIAEN IAYGDNSRVV PLDEIKEAAN AANIHSFIEG LPEKYNTQVG

    LKGAQLSGGQ KQRLAIARAL LQKPKILLLD EATSALDNDS EKVVQHALDK

    ARTGRTCLVV THRLSAIQNA DLIVVLHNGK IKEQGTHQEL LRNRDIYFKL

    VNAQSVQ

  • UCSC uc003suv.4; human. [Q2M3G0-4];




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