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Human ATG7 ELISA Kit

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  • Alternative name

    Human ATG12-activating enzyme E1 ATG7 ELISA Kit;Human Autophagy-related protein 7 ELISA Kit;Human APG7-LIKE ELISA Kit;Human hAGP7 ELISA Kit;Human ubiquitin-activating enzyme E1-like protein ELISA Kit;Human APG7L ELISA Kit;Human GSA7 ELISA Kit;Human autophagy related 7 ELISA Kit;Human ubiquitin-like modifier-activating enzyme ATG7 ELISA Kit;Human APG7 autophagy 7-like ELISA Kit;
  • Catalog
    E003939
  • species
    Human
  • GeneATG7
  • Other Species Mouse Atg7 ELISA KitChicken ATG7 ELISA KitRat Atg7 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human ATG7. No significant cross-reactivity or interference between human ATG7 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human ATG7 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O
  • Intended UseHuman ATG7 ELISA Kit allows for the in vitro quantitative determination of ATG7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ATG7 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ATG7 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ATG7 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ATG7 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Ubiquitin-like modifier-activating enzyme ATG7 Protein information
  • Uniprot ID ATG7_HUMAN
  • Uniprot AC O95352; B4E170; E9PB95; Q7L8L0; Q9BWP2; Q9UFH4;
  • UniGene Hs.38032;
  • GeneID 10533
  • KEGG hsa:10533;
  • Human Ubiquitin-like modifier-activating enzyme ATG7 Protein SEQUENCE

  • SEQUENCE 703 AA; 77960 MW; ABBD1A29A6C58356 CRC64;

    MAAATGDPGL SKLQFAPFSS ALDVGFWHEL TQKKLNEYRL DEAPKDIKGY

    YYNGDSAGLP ARLTLEFSAF DMSAPTPARC CPAIGTLYNT NTLESFKTAD

    KKLLLEQAAN EIWESIKSGT ALENPVLLNK FLLLTFADLK KYHFYYWFCY

    PALCLPESLP LIQGPVGLDQ RFSLKQIEAL ECAYDNLCQT EGVTALPYFL

    IKYDENMVLV SLLKHYSDFF QGQRTKITIG VYDPCNLAQY PGWPLRNFLV

    LAAHRWSSSF QSVEVVCFRD RTMQGARDVA HSIIFEVKLP EMAFSPDCPK

    AVGWEKNQKG GMGPRMVNLS ECMDPKRLAE SSVDLNLKLM CWRLVPTLDL

    DKVVSVKCLL LGAGTLGCNV ARTLMGWGVR HITFVDNAKI SYSNPVRQPL

    YEFEDCLGGG KPKALAAADR LQKIFPGVNA RGFNMSIPMP GHPVNFSSVT

    LEQARRDVEQ LEQLIESHDV VFLLMDTRES RWLPAVIAAS KRKLVINAAL

    GFDTFVVMRH GLKKPKQQGA GDLCPNHPVA SADLLGSSLF ANIPGYKLGC

    YFCNDVVAPG DSTRDRTLDQ QCTVSRPGLA VIAGALAVEL MVSVLQHPEG

    GYAIASSSDD RMNEPPTSLG LVPHQIRGFL SRFDNVLPVS LAFDKCTACS

    SKVLDQYERE GFNFLAKVFN SSHSFLEDLT GLTLLHQETQ AAEIWDMSDD

    ETI

  • UCSC uc003bwc.4; human. [O95352-1];


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