Human ATF5 ELISA Kit

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  • Alternative name

    Human cAMP-dependent transcription factor ATF-5 ELISA Kit;Human activating transcription factor 5 ELISA Kit;Human transcription factor ATFx ELISA Kit;Human ATFX ELISA Kit;Human HMFN0395 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-5 ELISA Kit;

  • Catalog
    E003930
  • species
    Human
  • GeneATF5
  • Other Species Mouse Atf5 ELISA KitRat Atf5 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ATF5. No significant cross-reactivity or interference between Human ATF5 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ATF5 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman ATF5 ELISA Kit allows for the in vitro quantitative determination of ATF5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATF5 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF5. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ATF5 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF5, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ATF5. You can calculate the concentration of Human ATF5 in the samples by comparing the OD of the samples to the standard curve.
  • Human Cyclic AMP-dependent transcription factor ATF-5 Protein information
  • Uniprot ID ATF5_HUMAN
  • Uniprot AC Q9Y2D1; B3KND3; Q9BSA1; Q9UNQ3;
  • UniGene Hs.9754;
  • GeneID 22809
  • KEGG hsa:22809;
  • Human Cyclic AMP-dependent transcription factor ATF-5 Protein SEQUENCE
  • SEQUENCE 282 AA; 30674 MW; DDB2F907CA0215A0 CRC64;

    MSLLATLGLE LDRALLPASG LGWLVDYGKL PPAPAPLAPY EVLGGALEGG

    LPVGGEPLAG DGFSDWMTER VDFTALLPLE PPLPPGTLPQ PSPTPPDLEA

    MASLLKKELE QMEDFFLDAP PLPPPSPPPL PPPPLPPAPS LPLSLPSFDL

    PQPPVLDTLD LLAIYCRNEA GQEEVGMPPL PPPQQPPPPS PPQPSRLAPY

    PHPATTRGDR KQKKRDQNKS AALRYRQRKR AEGEALEGEC QGLEARNREL

    KERAESVERE IQYVKDLLIE VYKARSQRTR SC

  • UCSC uc002prd.4; human.;




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