Human ATF2 ELISA Kit

Alternative name
Human cAMP-dependent transcription factor ATF-2 ELISA Kit;Human activating transcription factor 2 ELISA Kit;Human cyclic AMP-responsive element-binding protein 2 ELISA Kit;Human CREB-2 ELISA Kit;Human cAMP-responsive element-binding protein 2 ELISA Kit;Human HB16 ELISA Kit;Human histone acetyltransferase ATF2 ELISA Kit;Human cAMP response element-binding protein CRE-BP1 ELISA Kit;Human CREB2 ELISA Kit;Human CREBP1 ELISA Kit;Human CRE-BP1 ELISA Kit;Human TREB7 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-2 ELISA Kit;Human activating transcription factor 2 splice variant ATF2-var2 ELISA Kit;Human cAMP responsive element binding protein 2, formerly ELISA Kit;
Catalog
E003927
species
Human
GeneATF2
Other Species Mouse Atf2 ELISA Kit Chicken ATF2 ELISA Kit Rat Atf2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ATF2. No significant cross-reactivity or interference between Human ATF2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ATF2 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity9.375pg/ml
Intended UseHuman ATF2 ELISA Kit allows for the in vitro quantitative determination of ATF2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATF2 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ATF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ATF2. You can calculate the concentration of Human ATF2 in the samples by comparing the OD of the samples to the standard curve.
Human Cyclic AMP-dependent transcription factor ATF-2 Protein information
Uniprot ID ATF2_HUMAN
Uniprot AC Q3B7B7; Q4ZFU9; Q53RY2; Q8TAR1; Q96JT8;
UniGene Hs.592510;
GeneID 1386
KEGG hsa:1386;
Human Cyclic AMP-dependent transcription factor ATF-2 Protein SEQUENCE
SEQUENCE 505 AA; 54537 MW; 0190EEFAEC8891A7 CRC64;
MKFKLHVNSA RQYKDLWNMS DDKPFLCTAP GCGQRFTNED HLAVHKHKHE
MTLKFGPARN DSVIVADQTP TPTRFLKNCE EVGLFNELAS PFENEFKKAS
EDDIKKMPLD LSPLATPIIR SKIEEPSVVE TTHQDSPLPH PESTTSDEKE
VPLAQTAQPT SAIVRPASLQ VPNVLLTSSD SSVIIQQAVP SPTSSTVITQ
APSSNRPIVP VPGPFPLLLH LPNGQTMPVA IPASITSSNV HVPAAVPLVR
PVTMVPSVPG IPGPSSPQPV QSEAKMRLKA ALTQQHPPVT NGDTVKGHGS
GLVRTQSEES RPQSLQQPAT STTETPASPA HTTPQTQSTS GRRRRAANED
PDEKRRKFLE RNRAAASRCR QKRKVWVQSL EKKAEDLSSL NGQLQSEVTL
LRNEVAQLKQ LLLAHKDCPV TAMQKKSGYH TADKDDSSED ISVPSSPHTE
AIQHSSVSTS NGVSSTSKAE AVATSVLTQM ADQSTEPALS QIVMAPSSQS
QPSGS
UCSC uc002ujk.5; human. [P15336-1];

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Human ATF2 ELISA Kit