Human ATF1 ELISA Kit

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  • Alternative name

    Human cAMP-dependent transcription factor ATF-1 ELISA Kit;Human activating transcription factor 1 ELISA Kit;Human Protein TREB36 ELISA Kit;Human EWS-ATF1 ELISA Kit;Human FUS/ATF-1 ELISA Kit;Human TREB36 ELISA Kit;Human cyclic AMP-dependent transcription factor ATF-1 ELISA Kit;

  • Catalog
    E003926
  • species
    Human
  • GeneATF1
  • Other Species Mouse Atf1 ELISA KitBovine ATF1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Human ATF1. No significant cross-reactivity or interference between Human ATF1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Human ATF1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.938ng/mL
  • Intended UseHuman ATF1 ELISA Kit allows for the in vitro quantitative determination of ATF1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of ATF1 in human serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human ATF1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Human ATF1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human ATF1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human ATF1. You can calculate the concentration of Human ATF1 in the samples by comparing the OD of the samples to the standard curve.
  • Human Cyclic AMP-dependent transcription factor ATF-1 Protein information
  • Uniprot ID ATF1_HUMAN
  • Uniprot AC P18846; B4DRF9; P25168; Q9H4A8;
  • UniGene Hs.648565;
  • GeneID 466
  • KEGG hsa:466;
  • Human Cyclic AMP-dependent transcription factor ATF-1 Protein SEQUENCE
  • SEQUENCE 271 AA; 29233 MW; BAFECBFFB244FCED CRC64;

    MEDSHKSTTS ETAPQPGSAV QGAHISHIAQ QVSSLSESEE SQDSSDSIGS

    SQKAHGILAR RPSYRKILKD LSSEDTRGRK GDGENSGVSA AVTSMSVPTP

    IYQTSSGQYI AIAPNGALQL ASPGTDGVQG LQTLTMTNSG STQQGTTILQ

    YAQTSDGQQI LVPSNQVVVQ TASGDMQTYQ IRTTPSATSL PQTVVMTSPV

    TLTSQTTKTD DPQLKREIRL MKNREAAREC RRKKKEYVKC LENRVAVLEN

    QNKTLIEELK TLKDLYSNKS V

  • UCSC uc001rww.5; human. [P18846-1];


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