Human TMEM176B ELISA Kit

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  • Alternative name

    Human Protein LR8 ELISA Kit;Human LR8 ELISA Kit;Human MS4B2 ELISA Kit;Human transmembrane protein 176B ELISA Kit;Human LR8-like protein ELISA Kit;

  • Catalog
    E039236
  • species
    Human
  • GeneTMEM176B
  • Standard CurveHuman TMEM176B ELISA Kit
  • Other Species Mouse Tmem176b ELISA KitRat Tmem176b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Transmembrane Protein 176B (TMEM176B). No significant cross-reactivity or interference between Transmembrane Protein 176B (TMEM176B) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.64ng/mL.
  • Intended UseHuman TMEM176B ELISA Kit allows for the in vitro quantitative determination of TMEM176B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transmembrane Protein 176B (TMEM176B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transmembrane Protein 176B (TMEM176B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transmembrane Protein 176B (TMEM176B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transmembrane Protein 176B (TMEM176B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Transmembrane protein 176B Protein information
  • Uniprot ID T176B_HUMAN
  • Uniprot AC Q3YBM2; B2RDK2; D3DWZ7; E9PAV4; Q5BJI2; Q9BT42; Q9Y609;
  • UniGene Hs.647090; Hs.709505;
  • GeneID 28959
  • KEGG hsa:28959;
  • Human Transmembrane protein 176B Protein SEQUENCE
  • SEQUENCE 270 AA; 29056 MW; 6D27B2A384709FDD CRC64;

    MTQNTVIVNG VAMASRPSQP THVNVHIHQE SALTQLLKAG GSLKKFLFHP

    GDTVPSTARI GYEQLALGVT QILLGVVSCV LGVCLSLGPW TVLSASGCAF

    WAGSVVIAAG AGAIVHEKHP GKLAGYISSL LTLAGFATAM AAVVLCVNSF

    IWQTEPFLYI DTVCDRSDPV FPTTGYRWMR RSQENQWQKE ECRAYMQMLR

    KLFTAIRALF LAVCVLKVIV SLVSLGVGLR NLCGQSSQPL NEEGSEKRLL

    GENSVPPSPS REQTSTAIVL

  • UCSC uc003whu.5; human. [Q3YBM2-1];


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