Human ATXN2 ELISA Kit

Contact Us
  • Alternative name

    Human spinocerebellar ataxia type 2 protein ELISA Kit;Human trinucleotide repeat-containing gene 13 protein ELISA Kit;Human ATX2 ELISA Kit;Human SCA2 ELISA Kit;Human TNRC13 ELISA Kit;Human ataxin 2 ELISA Kit;Human ataxin-2 ELISA Kit;

  • Catalog
    E003909
  • species
    Human
  • GeneATXN2
  • Standard CurveHuman ATXN2 ELISA Kit
  • Other Species Mouse Atxn2 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATXN2 ELISA Kit allows for the in vitro quantitative determination of ATXN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Principle of the Assay: ATXN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATXN2 antibody and an ATXN2-HRP conjugate. The assay sample and buffer are incubated together with ATXN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN2 concentration since ATXN2 from samples and ATXN2-HRP conjugate compete for the anti-ATXN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN2 from the sample, fewer sites are left to bind ATXN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN2 concentration in each sample is interpolated from this standard curve.
  • Human Ataxin-2 Protein information
  • Uniprot ID ATX2_HUMAN
  • Uniprot AC Q99700; A6NLD4; Q24JQ7; Q6ZQZ7; Q99493;
  • UniGene Hs.732512; Hs.76253;
  • GeneID 6311
  • KEGG hsa:6311;
  • Human Ataxin-2 Protein SEQUENCE
  • SEQUENCE 1313 AA; 140283 MW; 40A2883FF9D5D118 CRC64;

    MRSAAAAPRS PAVATESRRF AAARWPGWRS LQRPARRSGR GGGGAAPGPY

    PSAAPPPPGP GPPPSRQSSP PSASDCFGSN GNGGGAFRPG SRRLLGLGGP

    PRPFVVLLLP LASPGAPPAA PTRASPLGAR ASPPRSGVSL ARPAPGCPRP

    ACEPVYGPLT MSLKPQQQQQ QQQQQQQQQQ QQQQQQQQPP PAAANVRKPG

    GSGLLASPAA APSPSSSSVS SSSATAPSSV VAATSGGGRP GLGRGRNSNK

    GLPQSTISFD GIYANMRMVH ILTSVVGSKC EVQVKNGGIY EGVFKTYSPK

    CDLVLDAAHE KSTESSSGPK REEIMESILF KCSDFVVVQF KDMDSSYAKR

    DAFTDSAISA KVNGEHKEKD LEPWDAGELT ANEELEALEN DVSNGWDPND

    MFRYNEENYG VVSTYDSSLS SYTVPLERDN SEEFLKREAR ANQLAEEIES

    SAQYKARVAL ENDDRSEEEK YTAVQRNSSE REGHSINTRE NKYIPPGQRN

    REVISWGSGR QNSPRMGQPG SGSMPSRSTS HTSDFNPNSG SDQRVVNGGV

    PWPSPCPSPS SRPPSRYQSG PNSLPPRAAT PTRPPSRPPS RPSRPPSHPS

    AHGSPAPVST MPKRMSSEGP PRMSPKAQRH PRNHRVSAGR GSISSGLEFV

    SHNPPSEAAT PPVARTSPSG GTWSSVVSGV PRLSPKTHRP RSPRQNSIGN

    TPSGPVLASP QAGIIPTEAV AMPIPAASPT PASPASNRAV TPSSEAKDSR

    LQDQRQNSPA GNKENIKPNE TSPSFSKAEN KGISPVVSEH RKQIDDLKKF

    KNDFRLQPSS TSESMDQLLN KNREGEKSRD LIKDKIEPSA KDSFIENSSS

    NCTSGSSKPN SPSISPSILS NTEHKRGPEV TSQGVQTSSP ACKQEKDDKE

    EKKDAAEQVR KSTLNPNAKE FNPRSFSQPK PSTTPTSPRP QAQPSPSMVG

    HQQPTPVYTQ PVCFAPNMMY PVPVSPGVQP LYPIPMTPMP VNQAKTYRAV

    PNMPQQRQDQ HHQSAMMHPA SAAGPPIAAT PPAYSTQYVA YSPQQFPNQP

    LVQHVPHYQS QHPHVYSPVI QGNARMMAPP THAQPGLVSS SATQYGAHEQ

    THAMYACPKL PYNKETSPSF YFAISTGSLA QQYAHPNATL HPHTPHPQPS

    ATPTGQQQSQ HGGSHPAPSP VQHHQHQAAQ ALHLASPQQQ SAIYHAGLAP

    TPPSMTPASN TQSPQNSFPA AQQTVFTIHP SHVQPAYTNP PHMAHVPQAH

    VQSGMVPSHP TAHAPMMLMT TQPPGGPQAA LAQSALQPIP VSTTAHFPYM

    THPSVQAHHQ QQL

  • UCSC uc001tsj.3; human. [Q99700-1];


Related products with Human ATXN2 ELISA Kit


tag

Inquiry About Human ATXN2 ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!