Human ATXN2 ELISA Kit

Alternative name
Human spinocerebellar ataxia type 2 protein ELISA Kit;Human trinucleotide repeat-containing gene 13 protein ELISA Kit;Human ATX2 ELISA Kit;Human SCA2 ELISA Kit;Human TNRC13 ELISA Kit;Human ataxin 2 ELISA Kit;Human ataxin-2 ELISA Kit;
Catalog
E003909
species
Human
GeneATXN2
Standard Curve
Other Species Mouse Atxn2 ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman ATXN2 ELISA Kit allows for the in vitro quantitative determination of ATXN2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the Assay: ATXN2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATXN2 antibody and an ATXN2-HRP conjugate. The assay sample and buffer are incubated together with ATXN2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATXN2 concentration since ATXN2 from samples and ATXN2-HRP conjugate compete for the anti-ATXN2 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATXN2 from the sample, fewer sites are left to bind ATXN2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATXN2 concentration in each sample is interpolated from this standard curve.
Human Ataxin-2 Protein information
Uniprot ID ATX2_HUMAN
Uniprot AC Q99700; A6NLD4; Q24JQ7; Q6ZQZ7; Q99493;
UniGene Hs.732512; Hs.76253;
GeneID 6311
KEGG hsa:6311;
Human Ataxin-2 Protein SEQUENCE
SEQUENCE 1313 AA; 140283 MW; 40A2883FF9D5D118 CRC64;
MRSAAAAPRS PAVATESRRF AAARWPGWRS LQRPARRSGR GGGGAAPGPY
PSAAPPPPGP GPPPSRQSSP PSASDCFGSN GNGGGAFRPG SRRLLGLGGP
PRPFVVLLLP LASPGAPPAA PTRASPLGAR ASPPRSGVSL ARPAPGCPRP
ACEPVYGPLT MSLKPQQQQQ QQQQQQQQQQ QQQQQQQQPP PAAANVRKPG
GSGLLASPAA APSPSSSSVS SSSATAPSSV VAATSGGGRP GLGRGRNSNK
GLPQSTISFD GIYANMRMVH ILTSVVGSKC EVQVKNGGIY EGVFKTYSPK
CDLVLDAAHE KSTESSSGPK REEIMESILF KCSDFVVVQF KDMDSSYAKR
DAFTDSAISA KVNGEHKEKD LEPWDAGELT ANEELEALEN DVSNGWDPND
MFRYNEENYG VVSTYDSSLS SYTVPLERDN SEEFLKREAR ANQLAEEIES
SAQYKARVAL ENDDRSEEEK YTAVQRNSSE REGHSINTRE NKYIPPGQRN
REVISWGSGR QNSPRMGQPG SGSMPSRSTS HTSDFNPNSG SDQRVVNGGV
PWPSPCPSPS SRPPSRYQSG PNSLPPRAAT PTRPPSRPPS RPSRPPSHPS
AHGSPAPVST MPKRMSSEGP PRMSPKAQRH PRNHRVSAGR GSISSGLEFV
SHNPPSEAAT PPVARTSPSG GTWSSVVSGV PRLSPKTHRP RSPRQNSIGN
TPSGPVLASP QAGIIPTEAV AMPIPAASPT PASPASNRAV TPSSEAKDSR
LQDQRQNSPA GNKENIKPNE TSPSFSKAEN KGISPVVSEH RKQIDDLKKF
KNDFRLQPSS TSESMDQLLN KNREGEKSRD LIKDKIEPSA KDSFIENSSS
NCTSGSSKPN SPSISPSILS NTEHKRGPEV TSQGVQTSSP ACKQEKDDKE
EKKDAAEQVR KSTLNPNAKE FNPRSFSQPK PSTTPTSPRP QAQPSPSMVG
HQQPTPVYTQ PVCFAPNMMY PVPVSPGVQP LYPIPMTPMP VNQAKTYRAV
PNMPQQRQDQ HHQSAMMHPA SAAGPPIAAT PPAYSTQYVA YSPQQFPNQP
LVQHVPHYQS QHPHVYSPVI QGNARMMAPP THAQPGLVSS SATQYGAHEQ
THAMYACPKL PYNKETSPSF YFAISTGSLA QQYAHPNATL HPHTPHPQPS
ATPTGQQQSQ HGGSHPAPSP VQHHQHQAAQ ALHLASPQQQ SAIYHAGLAP
TPPSMTPASN TQSPQNSFPA AQQTVFTIHP SHVQPAYTNP PHMAHVPQAH
VQSGMVPSHP TAHAPMMLMT TQPPGGPQAA LAQSALQPIP VSTTAHFPYM
THPSVQAHHQ QQL
UCSC uc001tsj.3; human. [Q99700-1];

Related products with Human ATXN2 ELISA Kit

anti-Ataxin 2 antibody

Category

Human ATXN2 ELISA Kit

Human spinocerebellar ataxia type 2 protein ELISA Kit;Human trinucleotide repeat-containing gene 13 protein ELISA Kit;Human ATX2 ELISA Kit;Human SCA2 ELISA Kit;Human TNRC13 ELISA Kit;Human ataxin 2 ELISA Kit;Human ataxin-2 ELISA Kit;