Human ARID5B ELISA Kit

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  • Alternative name

    Human ARID domain-containing protein 5B ELISA Kit;Human MRF1-like protein ELISA Kit;Human Modulator recognition factor 2 ELISA Kit;Human MRF-2 ELISA Kit;Human DESRT ELISA Kit;Human MRF2 ELISA Kit;Human AT-rich interaction domain 5B ELISA Kit;Human AT-rich interactive domain-containing protein 5B ELISA Kit;Human AT-rich interactive domain 5B (MRF1-like) ELISA Kit;Human modulator recognition factor 2 (MRF2) ELISA Kit;

  • Catalog
    E003890
  • species
    Human
  • GeneARID5B
  • Standard CurveHuman ARID5B ELISA Kit
  • Other Species Mouse Arid5b ELISA KitBovine ARID5B ELISA KitCanine ARID5B ELISA KitChicken ARID5B ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ARID5B. No significant cross-reactivity or interference between ARID5B and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ARID5B and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ARID5B ELISA Kit allows for the in vitro quantitative determination of ARID5B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: ARID5B ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ARID5B antibody and an ARID5B-HRP conjugate. The assay sample and buffer are incubated together with ARID5B-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ARID5B concentration since ARID5B from samples and ARID5B-HRP conjugate compete for the anti-ARID5B antibody binding site. Since the number of sites is limited, as more sites are occupied by ARID5B from the sample, fewer sites are left to bind ARID5B-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ARID5B concentration in each sample is interpolated from this standard curve.
  • Human AT-rich interactive domain-containing protein 5B Protein information
  • Uniprot ID ARI5B_HUMAN
  • Uniprot AC Q9H786;
  • UniGene Hs.535297;
  • GeneID 84159
  • KEGG hsa:84159;
  • Human AT-rich interactive domain-containing protein 5B Protein SEQUENCE
  • SEQUENCE 1188 AA; 132375 MW; 23B7C525B0055330 CRC64;

    MEPNSLQWVG SPCGLHGPYI FYKAFQFHLE GKPRILSLGD FFFVRCTPKD

    PICIAELQLL WEERTSRQLL SSSKLYFLPE DTPQGRNSDH GEDEVIAVSE

    KVIVKLEDLV KWVHSDFSKW RCGFHAGPVK TEALGRNGQK EALLKYRQST

    LNSGLNFKDV LKEKADLGED EEETNVIVLS YPQYCRYRSM LKRIQDKPSS

    ILTDQFALAL GGIAVVSRNP QILYCRDTFD HPTLIENESI CDEFAPNLKG

    RPRKKKPCPQ RRDSFSGVKD SNNNSDGKAV AKVKCEARSA LTKPKNNHNC

    KKVSNEEKPK VAIGEECRAD EQAFLVALYK YMKERKTPIE RIPYLGFKQI

    NLWTMFQAAQ KLGGYETITA RRQWKHIYDE LGGNPGSTSA ATCTRRHYER

    LILPYERFIK GEEDKPLPPI KPRKQENSSQ ENENKTKVSG TKRIKHEIPK

    SKKEKENAPK PQDAAEVSSE QEKEQETLIS QKSIPEPLPA ADMKKKIEGY

    QEFSAKPLAS RVDPEKDNET DQGSNSEKVA EEAGEKGPTP PLPSAPLAPE

    KDSALVPGAS KQPLTSPSAL VDSKQESKLC CFTESPESEP QEASFPSFPT

    TQPPLANQNE TEDDKLPAMA DYIANCTVKV DQLGSDDIHN ALKQTPKVLV

    VQSFDMFKDK DLTGPMNENH GLNYTPLLYS RGNPGIMSPL AKKKLLSQVS

    GASLSSSYPY GSPPPLISKK KLIARDDLCS SLSQTHHGQS TDHMAVSRPS

    VIQHVQSFRS KPSEERKTIN DIFKHEKLSR SDPHRCSFSK HHLNPLADSY

    VLKQEIQEGK DKLLEKRALP HSHMPSFLAD FYSSPHLHSL YRHTEHHLHN

    EQTSKYPSRD MYRESENSSF PSHRHQEKLH VNYLTSLHLQ DKKSAAAEAP

    TDDQPTDLSL PKNPHKPTGK VLGLAHSTTG PQESKGISQF QVLGSQSRDC

    HPKACRVSPM TMSGPKKYPE SLSRSGKPHH VRLENFRKME GMVHPILHRK

    MSPQNIGAAR PIKRSLEDLD LVIAGKKARA VSPLDPSKEV SGKEKASEQE

    SEGSKAAHGG HSGGGSEGHK LPLSSPIFPG LYSGSLCNSG LNSRLPAGYS

    HSLQYLKNQT VLSPLMQPLA FHSLVMQRGI FTSPTNSQQL YRHLAAATPV

    GSSYGDLLHN SIYPLAAINP QAAFPSSQLS SVHPSTKL

  • UCSC uc001jlt.3; human. [Q14865-1];




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