Human MAF ELISA Kit

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  • Alternative name

    Human proto-oncogene c-Maf ELISA Kit;Human V-maf musculoaponeurotic fibrosarcoma oncogene homolog ELISA Kit;Human AYGRP ELISA Kit;Human CCA4 ELISA Kit;Human CTRCT21 ELISA Kit;Human c-MAF ELISA Kit;Human MAF bZIP transcription factor ELISA Kit;Human transcription factor Maf ELISA Kit;Human Avian musculoaponeurotic fibrosarcoma (MAF) protooncogene ELISA Kit;Human T lymphocyte c-maf long form ELISA Kit;Human c-maf proto-oncogene ELISA Kit;Human v-maf avian musculoaponeurotic fibrosarcoma oncogene homolog ELISA Kit;

  • Catalog
    E038825
  • species
    Human
  • GeneMAF
  • Standard CurveHuman MAF ELISA Kit
  • Other Species Mouse Maf ELISA KitBovine MAF ELISA KitChicken MAF ELISA KitRat Maf ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAF. No significant cross-reactivity or interference between MAF and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAF and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 mug/mL.
  • Intended UseHuman MAF ELISA Kit allows for the in vitro quantitative determination of MAF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: MAF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-MAF antibody and an MAF-HRP conjugate. The assay sample and buffer are incubated together with MAF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAF concentration since MAF from samples and MAF-HRP conjugate compete for the anti-MAF antibody binding site. Since the number of sites is limited, as more sites are occupied by MAF from the sample, fewer sites are left to bind MAF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAF concentration in each sample is interpolated from this standard curve.
  • Human Transcription factor Maf Protein information
  • Uniprot ID MAF_HUMAN
  • Uniprot AC O75444; Q66I47; Q9UP93;
  • UniGene Hs.134859;
  • GeneID 4094
  • KEGG hsa:4094;
  • Human Transcription factor Maf Protein SEQUENCE
  • SEQUENCE 373 AA; 38492 MW; 566C2BC3E4A62762 CRC64;

    MASELAMSNS DLPTSPLAME YVNDFDLMKF EVKKEPVETD RIISQCGRLI

    AGGSLSSTPM STPCSSVPPS PSFSAPSPGS GSEQKAHLED YYWMTGYPQQ

    LNPEALGFSP EDAVEALISN SHQLQGGFDG YARGAQQLAA AAGAGAGASL

    GGSGEEMGPA AAVVSAVIAA AAAQSGAGPH YHHHHHHAAG HHHHPTAGAP

    GAAGSAAASA GGAGGAGGGG PASAGGGGGG GGGGGGGGAA GAGGALHPHH

    AAGGLHFDDR FSDEQLVTMS VRELNRQLRG VSKEEVIRLK QKRRTLKNRG

    YAQSCRFKRV QQRHVLESEK NQLLQQVDHL KQEISRLVRE RDAYKEKYEK

    LVSSGFRENG SSSDNPSSPE FFM

  • UCSC uc002ffm.4; human. [O75444-2];


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