Human TFAM ELISA Kit

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  • Alternative name

    Human MTTFA ELISA Kit;Human mitochondrial transcription factor 1 ELISA Kit;Human MTTF1 ELISA Kit;Human transcription factor 6 ELISA Kit;Human TCF-6 ELISA Kit;Human Transcription factor 6-like 2 ELISA Kit;Human TCF6 ELISA Kit;Human TCF6L2 ELISA Kit;Human MTDPS15 ELISA Kit;Human TCF6L1 ELISA Kit;Human TCF6L3 ELISA Kit;Human transcription factor A, mitochondrial ELISA Kit;Human mitochondrial transcription factor A ELISA Kit;Human transcription factor 6-like 1 ELISA Kit;Human transcription factor 6-like 2 (mitochondrial transcription factor) ELISA Kit;Human transcription factor 6-like 3 ELISA Kit;

  • Catalog
    E038791
  • species
    Human
  • GeneTFAM
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Transcription Factor A, Mitochondrial (TFAM). No significant cross-reactivity or interference between Transcription Factor A, Mitochondrial (TFAM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.124ng/mL.
  • Intended UseHuman TFAM ELISA Kit allows for the in vitro quantitative determination of TFAM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transcription Factor A, Mitochondrial (TFAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transcription Factor A, Mitochondrial (TFAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transcription Factor A, Mitochondrial (TFAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transcription Factor A, Mitochondrial (TFAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Transcription factor A, mitochondrial Protein information
  • Uniprot ID TFAM_HUMAN
  • Uniprot AC Q00059; A8MRB2; A9QXC6; B5BU05; Q5U0C6;
  • UniGene Hs.594250;
  • GeneID 7019
  • KEGG hsa:7019;
  • Human Transcription factor A, mitochondrial Protein SEQUENCE
  • SEQUENCE 246 AA; 29097 MW; 81F00CFA12DA4924 CRC64;

    MAFLRSMWGV LSALGRSGAE LCTGCGSRLR SPFSFVYLPR WFSSVLASCP

    KKPVSSYLRF SKEQLPIFKA QNPDAKTTEL IRRIAQRWRE LPDSKKKIYQ

    DAYRAEWQVY KEEISRFKEQ LTPSQIMSLE KEIMDKHLKR KAMTKKKELT

    LLGKPKRPRS AYNVYVAERF QEAKGDSPQE KLKTVKENWK NLSDSEKELY

    IQHAKEDETR YHNEMKSWEE QMIEVGRKDL LRRTIKKQRK YGAEEC

  • UCSC uc001jkf.5; human. [Q00059-1];



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