Human TRACP-5b ELISA Kit

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  • Catalog
    E038712
  • species
    Human
  • GeneTRACP-5b
  • Standard CurveHuman TRACP-5b ELISA Kit
  • Other Species Mouse TRACP-5b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TRACP-5b . No significant cross-reactivity or interference between TRACP-5b and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.188mIU/ml
  • Intended UseHuman TRACP-5b ELISA Kit allows for the in vitro quantitative determination of TRACP-5b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- TRACP- 5bantibody was pre-coated onto 96-well plates. And the biotin conjugated anti- TRACP-5b antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the TRACP-5b amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of TRACP-5b can be calculated.


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