Human ASTL ELISA Kit

Alternative name
Human oocyte astacin ELISA Kit;Human ovastacin ELISA Kit;Human SAS1B ELISA Kit;Human astacin like metalloendopeptidase ELISA Kit;Human astacin-like metalloendopeptidase ELISA Kit;Human astacin-like metallo-endopeptidase (M12 family) ELISA Kit;Human astacin-like metalloendopeptidase (M12 family) ELISA Kit;Human sperm acrosomal SLLP1 binding ELISA Kit;
Catalog
E003863
species
Human
GeneASTL
Standard Curve
Other Species Mouse Astl ELISA Kit Chicken ASTL ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ASTL. No significant cross-reactivity or interference between ASTL and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human ASTL is typically less than 0.060ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by addi
Intended UseHuman ASTL ELISA Kit allows for the in vitro quantitative determination of ASTL , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASTL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ASTL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASTL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ASTL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Astacin-like metalloendopeptidase Protein information
Uniprot ID ASTL_HUMAN
Uniprot AC Q6HA08; Q3KNT0;
UniGene Hs.447993;
GeneID 431705
KEGG hsa:431705;
Human Astacin-like metalloendopeptidase Protein SEQUENCE
SEQUENCE 431 AA; 45936 MW; 6E1FD4653506AFC1 CRC64;
MEGVGGLWPW VLGLLSLPGV ILGAPLASSC AGACGTSFPD GLTPEGTQAS
GDKDIPAINQ GLILEETPES SFLIEGDIIR PSPFRLLSAT SNKWPMGGSG
VVEVPFLLSS KYDEPSRQVI LEALAEFERS TCIRFVTYQD QRDFISIIPM
YGCFSSVGRS GGMQVVSLAP TCLQKGRGIV LHELMHVLGF WHEHTRADRD
RYIRVNWNEI LPGFEINFIK SQSSNMLTPY DYSSVMHYGR LAFSRRGLPT
ITPLWAPSVH IGQRWNLSAS DITRVLKLYG CSPSGPRPRG RGSHAHSTGR
SPAPASLSLQ RLLEALSAES RSPDPSGSSA GGQPVPAGPG ESPHGWESPA
LKKLSAEASA RQPQTLASSP RSRPGAGAPG VAQEQSWLAG VSTKPTVPSS
EAGIQPVPVQ GSPALPGGCV PRNHFKGMSE D
UCSC uc010yui.2; human.;

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Human ASTL ELISA Kit