Human TOP2 ELISA Kit

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  • Alternative name

    DNA topoisomerase 2-beta ELISA KIT; DNA topoisomerase II, beta isozymeTOP2B ELISA KIT;

  • Catalog
    E038604
  • species
    Human
  • GeneTOP2
  • Standard CurveHuman TOP2 ELISA Kit
  • Other Species Mouse TOP2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Topoisomerase II (TOP2). No significant cross-reactivity or interference between Topoisomerase II (TOP2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.237ng/mL.
  • Intended UseHuman TOP2 ELISA Kit allows for the in vitro quantitative determination of TOP2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Topoisomerase II (TOP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Topoisomerase II (TOP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Topoisomerase II (TOP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Topoisomerase II (TOP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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