Human DARS ELISA Kit

Alternative name
Human aspartyl-tRNA synthetase ELISA Kit;Human aspRS ELISA Kit;Human cell proliferation-inducing gene 40 protein ELISA Kit;Human HBSL ELISA Kit;Human aspartate--tRNA ligase, cytoplasmic ELISA Kit;Human aspartate tRNA ligase 1, cytoplasmic ELISA Kit;Human aspartyl-tRNA synthetase, cytoplasmic ELISA Kit;Human testicular tissue protein Li 192 ELISA Kit;
Catalog
E003849
species
Human
GeneDARS
Standard Curve
Other Species Mouse Dars ELISA Kit Bovine DARS ELISA Kit Rat Dars ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of DARS. No significant cross-reactivity or interference between DARS and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DARS and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman DARS ELISA Kit allows for the in vitro quantitative determination of DARS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyEpigenetics and Nuclear Signaling
Product Description
specificalIntended Uses: This DARS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human DARS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: DARS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DARS antibody and an DARS-HRP conjugate. The assay sample and buffer are incubated together with DARS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DARS concentration since DARS from samples and DARS-HRP conjugate compete for the anti-DARS antibody binding site. Since the number of sites is limited, as more sites are occupied by DARS from the sample, fewer sites are left to bind DARS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DARS concentration in each sample is interpolated from this standard curve.
Human Aspartate--tRNA ligase, cytoplasmic Protein information
Uniprot ID SYDC_HUMAN
Uniprot AC Q9BW52;
UniGene Hs.503787; Hs.595819;
GeneID 1615
KEGG hsa:1615;
Human Aspartate--tRNA ligase, cytoplasmic Protein SEQUENCE
SEQUENCE 501 AA; 57136 MW; B181572DF0AF5F94 CRC64;
MPSASASRKS QEKPREIMDA AEDYAKERYG ISSMIQSQEK PDRVLVRVRD
LTIQKADEVV WVRARVHTSR AKGKQCFLVL RQQQFNVQAL VAVGDHASKQ
MVKFAANINK ESIVDVEGVV RKVNQKIGSC TQQDVELHVQ KIYVISLAEP
RLPLQLDDAV RPEAEGEEEG RATVNQDTRL DNRVIDLRTS TSQAVFRLQS
GICHLFRETL INKGFVEIQT PKIISAASEG GANVFTVSYF KNNAYLAQSP
QLYKQMCICA DFEKVFSIGP VFRAEDSNTH RHLTEFVGLD IEMAFNYHYH
EVMEEIADTM VQIFKGLQER FQTEIQTVNK QFPCEPFKFL EPTLRLEYCE
ALAMLREAGV EMGDEDDLST PNEKLLGHLV KEKYDTDFYI LDKYPLAVRP
FYTMPDPRNP KQSNSYDMFM RGEEILSGAQ RIHDPQLLTE RALHHGIDLE
KIKAYIDSFR FGAPPHAGGG IGLERVTMLF LGLHNVRQTS MFPRDPKRLT
P
UCSC uc002tux.2; human. [P14868-1];

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Human DARS ELISA Kit