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Human ASPH ELISA Kit

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  • Alternative name

    Human aspartate beta-hydroxylase ELISA Kit;Human ASP beta-hydroxylase ELISA Kit;Human peptide-aspartate beta-dioxygenase ELISA Kit;Human BAH ELISA Kit;Human AAH ELISA Kit;Human CASQ2BP1 ELISA Kit;Human FDLAB ELISA Kit;Human HAAH ELISA Kit;Human JCTN ELISA Kit;Human junctin ELISA Kit;Human aspartyl/asparaginyl beta-hydroxylase ELISA Kit;Human A beta H-J-J ELISA Kit;Human cardiac junctin ELISA Kit;Human humbug ELISA Kit;Human junctate ELISA Kit;
  • Catalog
    E003839
  • species
    Human
  • GeneASPH
  • Standard CurveHuman ASPH ELISA Kit
  • Other Species Human ASPH CT ELISA KitHuman ASPH-CT ELISA KitMouse Asph ELISA KitMouse ASPH-CT ELISA KitBovine ASPH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Aspartate Beta Hydroxylase (ASPH). No significant cross-reactivity or interference between Aspartate Beta Hydroxylase (ASPH) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman ASPH ELISA Kit allows for the in vitro quantitative determination of ASPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Aspartate Beta Hydroxylase (ASPH). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Aspartate Beta Hydroxylase (ASPH). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Aspartate Beta Hydroxylase (ASPH), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Aspartate Beta Hydroxylase (ASPH) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Aspartyl/asparaginyl beta-hydroxylase Protein information
  • Uniprot ID ASPH_HUMAN
  • Uniprot AC Q6NXR7; Q8TB28; Q9H291; Q9H2C4; Q9NRI0; Q9NRI1; Q9Y4J0;
  • UniGene Hs.332422;
  • GeneID 444
  • KEGG hsa:444;
  • Human Aspartyl/asparaginyl beta-hydroxylase Protein SEQUENCE

  • SEQUENCE 758 AA; 85863 MW; 4AE56D1D8DF0AF0C CRC64;

    MAQRKNAKSS GNSSSSGSGS GSTSAGSSSP GARRETKHGG HKNGRKGGLS

    GTSFFTWFMV IALLGVWTSV AVVWFDLVDY EEVLGKLGIY DADGDGDFDV

    DDAKVLLGLK ERSTSEPAVP PEEAEPHTEP EEQVPVEAEP QNIEDEAKEQ

    IQSLLHEMVH AEHVEGEDLQ QEDGPTGEPQ QEDDEFLMAT DVDDRFETLE

    PEVSHEETEH SYHVEETVSQ DCNQDMEEMM SEQENPDSSE PVVEDERLHH

    DTDDVTYQVY EEQAVYEPLE NEGIEITEVT APPEDNPVED SQVIVEEVSI

    FPVEEQQEVP PETNRKTDDP EQKAKVKKKK PKLLNKFDKT IKAELDAAEK

    LRKRGKIEEA VNAFKELVRK YPQSPRARYG KAQCEDDLAE KRRSNEVLRG

    AIETYQEVAS LPDVPADLLK LSLKRRSDRQ QFLGHMRGSL LTLQRLVQLF

    PNDTSLKNDL GVGYLLIGDN DNAKKVYEEV LSVTPNDGFA KVHYGFILKA

    QNKIAESIPY LKEGIESGDP GTDDGRFYFH LGDAMQRVGN KEAYKWYELG

    HKRGHFASVW QRSLYNVNGL KAQPWWTPKE TGYTELVKSL ERNWKLIRDE

    GLAVMDKAKG LFLPEDENLR EKGDWSQFTL WQQGRRNENA CKGAPKTCTL

    LEKFPETTGC RRGQIKYSIM HPGTHVWPHT GPTNCRLRMH LGLVIPKEGC

    KIRCANETKT WEEGKVLIFD DSFEHEVWQD ASSFRLIFIV DVWHPELTPQ

    QRRSLPAI

  • UCSC uc003xuj.4; human. [Q12797-1];


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