Human ACTA2 ELISA Kit

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  • Alternative name

    Human Alpha-actin-2 ELISA Kit;Human cell growth-inhibiting gene 46 protein ELISA Kit;Human ACTSA ELISA Kit;Human ACTVS ELISA Kit;Human AAT6 ELISA Kit;Human MYMY5 ELISA Kit;Human actin, alpha 2, smooth muscle, aorta ELISA Kit;Human actin, aortic smooth muscle ELISA Kit;Human alpha-cardiac actin ELISA Kit;

  • Catalog
    E000383
  • species
    Human
  • GeneACTA2
  • Standard CurveHuman ACTA2 ELISA Kit
  • Other Species Mouse Acta2 ELISA KitBovine ACTA2 ELISA KitChicken ACTA2 ELISA KitRabbit ACTA2 ELISA KitRat Acta2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Actin Alpha 2, Smooth Muscle (ACTa2). No significant cross-reactivity or interference between Actin Alpha 2, Smooth Muscle (ACTa2) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 3.0ng/mL.
  • Intended UseHuman ACTA2 ELISA Kit allows for the in vitro quantitative determination of ACTA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 2, Smooth Muscle (ACTa2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 2, Smooth Muscle (ACTa2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 2, Smooth Muscle (ACTa2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Actin, aortic smooth muscle Protein information
  • Uniprot ID ACTA_HUMAN
  • Uniprot AC P62736; B2R8A4; P03996; P04108; Q6FI19;
  • UniGene Hs.500483;
  • GeneID 59
  • KEGG hsa:59;
  • Human Actin, aortic smooth muscle Protein SEQUENCE
  • SEQUENCE 377 AA; 42009 MW; 2D0543262DB35CA5 CRC64;

    MCEEEDSTAL VCDNGSGLCK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG

    QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH SFYNELRVAP

    EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT

    TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY

    SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI

    TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV

    LSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS

    LSTFQQMWIS KQEYDEAGPS IVHRKCF

  • UCSC uc001kfp.4; human.;




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