Human TSHB ELISA Kit

Alternative name
Human Thyroid-stimulating hormone subunit beta ELISA Kit;Human TSH-B ELISA Kit;Human TSH-BETA ELISA Kit;Human thyrotropin beta chain ELISA Kit;Human thyroid stimulating hormone beta ELISA Kit;Human thyrotropin subunit beta ELISA Kit;
Catalog
E038295
species
Human
GeneTSHB
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188mIU/mL
Intended UseHuman TSHB ELISA Kit allows for the in vitro quantitative determination of TSHB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human TSHBantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human TSHB antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human TSHB amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman TSHBcan be calculated.
Human Thyrotropin subunit beta Protein information
Uniprot ID TSHB_HUMAN
Uniprot AC P01222; B1AKP0; Q16163;
UniGene Hs.406687;
GeneID 7252
KEGG hsa:7252;
Human Thyrotropin subunit beta Protein SEQUENCE
SEQUENCE 138 AA; 15639 MW; 6A367538D8393DB7 CRC64;
MTALFLMSML FGLTCGQAMS FCIPTEYTMH IERRECAYCL TINTTICAGY
CMTRDINGKL FLPKYALSQD VCTYRDFIYR TVEIPGCPLH VAPYFSYPVA
LSCKCGKCNT DYSDCIHEAI KTNYCTKPQK SYLVGFSV
UCSC uc001efs.2; human. [P01222-1];

Human TSHB ELISA Kit

Human Thyroid-stimulating hormone subunit beta ELISA Kit;Human TSH-B ELISA Kit;Human TSH-BETA ELISA Kit;Human thyrotropin beta chain ELISA Kit;Human thyroid stimulating hormone beta ELISA Kit;Human thyrotropin subunit beta ELISA Kit;

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