Human ASNS ELISA Kit

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  • Alternative name

    Human Cell cycle control protein TS11 ELISA Kit;Human glutamine-dependent asparagine synthetase ELISA Kit;Human TS11 ELISA Kit;Human ASNSD ELISA Kit;Human asparagine synthetase (glutamine-hydrolyzing) ELISA Kit;Human asparagine synthetase [glutamine-hydrolyzing] ELISA Kit;Human TS11 cell cycle control protein ELISA Kit;

  • Catalog
    E003821
  • species
    Human
  • GeneASNS
  • Standard CurveHuman ASNS ELISA Kit
  • Other Species Mouse Asns ELISA KitBovine ASNS ELISA KitChicken ASNS ELISA KitHamster ASNS ELISA KitRat Asns ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ASNS. No significant cross-reactivity or interference between ASNS and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of ASNS is typically less than 0.32ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two
  • Intended UseHuman ASNS ELISA Kit allows for the in vitro quantitative determination of ASNS , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to ASNS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ASNS. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ASNS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ASNS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Asparagine synthetase [glutamine-hydrolyzing] Protein information
  • Uniprot ID ASNS_HUMAN
  • Uniprot AC Q15666; Q549T9; Q96HD0;
  • UniGene Hs.489207;
  • GeneID 440
  • KEGG hsa:440;
  • Human Asparagine synthetase [glutamine-hydrolyzing] Protein SEQUENCE
  • SEQUENCE 561 AA; 64370 MW; 3E9B75E21D799FBE CRC64;

    MCGIWALFGS DDCLSVQCLS AMKIAHRGPD AFRFENVNGY TNCCFGFHRL

    AVVDPLFGMQ PIRVKKYPYL WLCYNGEIYN HKKMQQHFEF EYQTKVDGEI

    ILHLYDKGGI EQTICMLDGV FAFVLLDTAN KKVFLGRDTY GVRPLFKAMT

    EDGFLAVCSE AKGLVTLKHS ATPFLKVEPF LPGHYEVLDL KPNGKVASVE

    MVKYHHCRDV PLHALYDNVE KLFPGFEIET VKNNLRILFN NAVKKRLMTD

    RRIGCLLSGG LDSSLVAATL LKQLKEAQVQ YPLQTFAIGM EDSPDLLAAR

    KVADHIGSEH YEVLFNSEEG IQALDEVIFS LETYDITTVR ASVGMYLISK

    YIRKNTDSVV IFSGEGSDEL TQGYIYFHKA PSPEKAEEES ERLLRELYLF

    DVLRADRTTA AHGLELRVPF LDHRFSSYYL SLPPEMRIPK NGIEKHLLRE

    TFEDSNLIPK EILWRPKEAF SDGITSVKNS WFKILQEYVE HQVDDAMMAN

    AAQKFPFNTP KTKEGYYYRQ VFERHYPGRA DWLSHYWMPK WINATDPSAR

    TLTHYKSAVK A

  • UCSC uc003uot.5; human. [P08243-1];


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