Human ACTA1 ELISA Kit

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  • Alternative name

    Human Alpha-actin-1 ELISA Kit;Human ACTA ELISA Kit;Human ASMA ELISA Kit;Human CFTD ELISA Kit;Human CFTD1 ELISA Kit;Human CFTDM ELISA Kit;Human MPFD ELISA Kit;Human NEM1 ELISA Kit;Human NEM2 ELISA Kit;Human NEM3 ELISA Kit;Human SHPM ELISA Kit;Human actin, alpha 1, skeletal muscle ELISA Kit;Human actin, alpha skeletal muscle ELISA Kit;Human nemaline myopathy type 3 ELISA Kit;

  • Catalog
    E000382
  • species
    Human
  • GeneACTA1
  • Standard CurveHuman ACTA1 ELISA Kit
  • Other Species Mouse Acta1 ELISA KitBovine ACTA1 ELISA KitChicken ACTA1 ELISA KitPorcine ACTA1 ELISA KitRabbit ACTA1 ELISA KitRat Acta1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Actin Alpha 1, Skeletal Muscle (ACTa1). No significant cross-reactivity or interference between Actin Alpha 1, Skeletal Muscle (ACTa1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.57ng/mL.
  • Intended UseHuman ACTA1 ELISA Kit allows for the in vitro quantitative determination of ACTA1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Alpha 1, Skeletal Muscle (ACTa1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Alpha 1, Skeletal Muscle (ACTa1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Alpha 1, Skeletal Muscle (ACTa1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Actin, alpha skeletal muscle Protein information
  • Uniprot ID ACTS_HUMAN
  • Uniprot AC P68133; P02568; P99020; Q5T8M9;
  • UniGene Hs.1288;
  • GeneID 58
  • KEGG hsa:58;
  • Human Actin, alpha skeletal muscle Protein SEQUENCE
  • SEQUENCE 377 AA; 42051 MW; DF2A3A046346A179 CRC64;

    MCDEDETTAL VCDNGSGLVK AGFAGDDAPR AVFPSIVGRP RHQGVMVGMG

    QKDSYVGDEA QSKRGILTLK YPIEHGIITN WDDMEKIWHH TFYNELRVAP

    EEHPTLLTEA PLNPKANREK MTQIMFETFN VPAMYVAIQA VLSLYASGRT

    TGIVLDSGDG VTHNVPIYEG YALPHAIMRL DLAGRDLTDY LMKILTERGY

    SFVTTAEREI VRDIKEKLCY VALDFENEMA TAASSSSLEK SYELPDGQVI

    TIGNERFRCP ETLFQPSFIG MESAGIHETT YNSIMKCDID IRKDLYANNV

    MSGGTTMYPG IADRMQKEIT ALAPSTMKIK IIAPPERKYS VWIGGSILAS

    LSTFQQMWIT KQEYDEAGPS IVHRKCF

  • UCSC uc001htm.4; human.;


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