SpecificityThis assay has high sensitivity and excellent specificity for detection of human thymosin beta4. No significant cross-reactivity or interference between human thymosin beta4 and analogues was observed.
SensitivityThe minimum detectable dose of human thymosin beta4 is typically less than 19.5 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determin
Intended UseHuman TMSB4X ELISA Kit allows for the in vitro quantitative determination of TMSB4X , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for thymosin beta4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any thymosin beta4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for thymosin beta4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of thymosin beta4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Hematopoietic system regulatory peptide Protein information
Uniprot ID
TYB4_HUMAN
Uniprot AC
P62328; P01253; P01254; Q546P5; Q63576; Q9UE55;
UniGene
Hs.437277; Hs.703237;
GeneID
7114
KEGG
hsa:7114;
Human Hematopoietic system regulatory peptide Protein SEQUENCE