Human TDG ELISA Kit

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  • Alternative name

    Human Thymine-DNA glycosylase ELISA Kit;Human hTDG ELISA Kit;Human thymine DNA glycosylase ELISA Kit;Human G/T mismatch-specific thymine DNA glycosylase ELISA Kit;

  • Catalog
    E038175
  • species
    Human
  • GeneTDG
  • Standard CurveHuman TDG ELISA Kit
  • Other Species Mouse Tdg ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Thymine DNA Glycosylase (TDG). No significant cross-reactivity or interference between Thymine DNA Glycosylase (TDG) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.121ng/mL.
  • Intended UseHuman TDG ELISA Kit allows for the in vitro quantitative determination of TDG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymine DNA Glycosylase (TDG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymine DNA Glycosylase (TDG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymine DNA Glycosylase (TDG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymine DNA Glycosylase (TDG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human G/T mismatch-specific thymine DNA glycosylase Protein information
  • Uniprot ID TDG_HUMAN
  • Uniprot AC Q13569; Q8IUZ6; Q8IZM3;
  • UniGene Hs.584809;
  • GeneID 6996
  • KEGG hsa:6996;
  • Human G/T mismatch-specific thymine DNA glycosylase Protein SEQUENCE
  • SEQUENCE 410 AA; 46053 MW; 33752B26EBC789AE CRC64;

    MEAENAGSYS LQQAQAFYTF PFQQLMAEAP NMAVVNEQQM PEEVPAPAPA

    QEPVQEAPKG RKRKPRTTEP KQPVEPKKPV ESKKSGKSAK SKEKQEKITD

    TFKVKRKVDR FNGVSEAELL TKTLPDILTF NLDIVIIGIN PGLMAAYKGH

    HYPGPGNHFW KCLFMSGLSE VQLNHMDDHT LPGKYGIGFT NMVERTTPGS

    KDLSSKEFRE GGRILVQKLQ KYQPRIAVFN GKCIYEIFSK EVFGVKVKNL

    EFGLQPHKIP DTETLCYVMP SSSARCAQFP RAQDKVHYYI KLKDLRDQLK

    GIERNMDVQE VQYTFDLQLA QEDAKKMAVK EEKYDPGYEA AYGGAYGENP

    CSSEPCGFSS NGLIESVELR GESAFSGIPN GQWMTQSFTD QIPSFSNHCG

    TQEQEEESHA

  • UCSC uc001tkg.4; human.;


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