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Alternative name
Thromboxane A2 receptor ELISA KIT; Prostanoid TP receptorTBXA2R ELISA KIT; TXA2-R ELISA KIT
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Catalog
E038117
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species
Human
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GeneTX A2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of TXA2. No significant cross-reactivity or interference between TXA2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete thecross-reactivity detection between TXA2 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseHuman TX A2 ELISA Kit allows for the in vitro quantitative determination of TX A2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCardiovascular
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Product Description
specificalPrinciple of the assay: TXA2 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TXA2. Standards or samples are then added to the microtiter plate wells and TXA2 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TXA2 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TXA2 are added to each well to "sandwich" the TXA2 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TXA2 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TXA2 concentration in each sample is interpolated from this standard curve.
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