Human THOP1 ELISA Kit

Alternative name
Human endopeptidase 24.15 ELISA Kit;Human MP78 ELISA Kit;Human EP24.15 ELISA Kit;Human MEPD_HUMAN ELISA Kit;Human TOP ELISA Kit;Human thimet oligopeptidase 1 ELISA Kit;Human thimet oligopeptidase ELISA Kit;
Catalog
E037977
species
Human
GeneTHOP1
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseHuman THOP1 ELISA Kit allows for the in vitro quantitative determination of THOP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human THOP1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human THOP1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human THOP1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human THOP1 can be calculated.
Human Thimet oligopeptidase Protein information
Uniprot ID THOP1_HUMAN
Uniprot AC P52888; B3KSE2; Q9UCB3;
UniGene Hs.78769;
GeneID 7064
KEGG hsa:7064;
Human Thimet oligopeptidase Protein SEQUENCE
SEQUENCE 689 AA; 78840 MW; 092D53DD63B322DE CRC64;
MKPPAACAGD MADAASPCSV VNDLRWDLSA QQIEERTREL IEQTKRVYDQ
VGTQEFEDVS YESTLKALAD VEVTYTVQRN ILDFPQHVSP SKDIRTASTE
ADKKLSEFDV EMSMREDVYQ RIVWLQEKVQ KDSLRPEAAR YLERLIKLGR
RNGLHLPRET QENIKRIKKK LSLLCIDFNK NLNEDTTFLP FTLQELGGLP
EDFLNSLEKM EDGKLKVTLK YPHYFPLLKK CHVPETRRKV EEAFNCRCKE
ENCAILKELV TLRAQKSRLL GFHTHADYVL EMNMAKTSQT VATFLDELAQ
KLKPLGEQER AVILELKRAE CERRGLPFDG RIRAWDMRYY MNQVEETRYC
VDQNLLKEYF PVQVVTHGLL GIYQELLGLA FHHEEGASAW HEDVRLYTAR
DAASGEVVGK FYLDLYPREG KYGHAACFGL QPGCLRQDGS RQIAIAAMVA
NFTKPTADAP SLLQHDEVET YFHEFGHVMH QLCSQAEFAM FSGTHVERDF
VEAPSQMLEN WVWEQEPLLR MSRHYRTGSA VPRELLEKLI ESRQANTGLF
NLRQIVLAKV DQALHTQTDA DPAEEYARLC QEILGVPATP GTNMPATFGH
LAGGYDAQYY GYLWSEVYSM DMFHTRFKQE GVLNSKVGMD YRSCILRPGG
SEDASAMLRR FLGRDPKQDA FLLSKGLQVG GCEPEPQVC
UCSC uc002lwj.4; human. [P52888-1];

Category

Human THOP1 ELISA Kit

Human endopeptidase 24.15 ELISA Kit;Human MP78 ELISA Kit;Human EP24.15 ELISA Kit;Human MEPD_HUMAN ELISA Kit;Human TOP ELISA Kit;Human thimet oligopeptidase 1 ELISA Kit;Human thimet oligopeptidase ELISA Kit;