Intended UseHuman TGF-beta1 ELISA Kit allows for the in vitro quantitative determination of TGF-beta1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalProduct Information: TGF-beta 1 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human TGF-beta 1 in serum, plasma, cell culture supernatants & human urine. Detection Range: 15.6 pg/ml - 1000 pg/ml. 100 assays.
Background: TGF-beta1 is a multifunctional cytokine involved in cell growth, differentiation and inflammatory pathways. Biobool's human TGF-beta1 ELISA Kit is based on the standard principle of a sandwich enzyme-linked immunosorbent assay. A mouse monoclonal antibody specific for human TGF-beta1 is coated on a 96-well plate. Standards and test samples are added to the wells and TGF-beta1 present in a sample is bound by the immobilized antibody. A biotinylated polyclonal antibody from goat specific for TGF-beta1 is added subsequently. After washing away the unbound biotinylated antibody with PBS or TBS buffer, avidin-Biotin-Peroxidase Complex is added to the wells. The wells are again washed with PBS or TBS buffer to remove the unbound conjugates. HRP substrate TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue product that changes into yellow after adding acidic stop solution. The density of yellow color is proportional to the human TGF-beta1 captured onto the plate. This ELISA kit shows <1% cross-reactivity with TGF-beta2, TGF-beta3 and TGF-beta5. Detection Range: 15.6 pg/ml - 1000 pg/ml. Sensitivity: < 1 pg/ml.
Summary: Detection method- Absorbance (450 nm)
Application- Quantitative protein detection, establishing normal range etc.