Human TFF3 ELISA Kit

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  • Alternative name

    Human Intestinal trefoil factor ELISA Kit;Human hITF ELISA Kit;Human polypeptide P1.B ELISA Kit;Human hP1.B ELISA Kit;Human ITF ELISA Kit;Human TFI ELISA Kit;Human P1B ELISA Kit;Human trefoil factor 3 ELISA Kit;Human trefoil factor 3 (intestinal) ELISA Kit;

  • Catalog
    E037902
  • species
    Human
  • GeneTFF3
  • Other Species Mouse Tff3 ELISA KitBovine TFF3 ELISA KitCanine TFF3 ELISA KitPorcine TFF3 ELISA KitRat Tff3 ELISA Kit
  • SpecificityNatural and recombinant human TFF3
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 10pg/ml
  • Intended UseHuman TFF3 ELISA Kit allows for the in vitro quantitative determination of TFF3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human TFF3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for TFF3 has been precoated onto 96-well plates. Standards(E.coli, E22-Y80) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for TFF3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human TFF3 amount of sample captured in plate. Background: Trefoil factor 3 is a protein that in humans is encoded by the TFF3 gene. It is mapped to 21q22.3. Members of the trefoil family can interact with mucins and have an influence on mucus viscosity. They also promote migration of epithelial cells, are linked to antiapoptosis, induce cell scattering, trigger chemotaxis, and participate in immune responses. This gene is a marker of columnar epithelium and is expressed in a variety of tissues including goblet cells of the intestines and colon. It has been found that TFF3 is the most highly upregulated gene in 19 poorly differentiated endometrioid endometrial carcinomas (G3-EECs) compared with normal endometrium biopsies.
  • Human Trefoil factor 3 Protein information
  • Uniprot ID TFF3_HUMAN
  • Uniprot AC Q07654; E9PBB5; Q96NX0; Q9UDA5;
  • UniGene Hs.82961;
  • GeneID 7033
  • KEGG hsa:7033;
  • Human Trefoil factor 3 Protein SEQUENCE
  • SEQUENCE 80 AA; 8641 MW; 8E117A58C0342013 CRC64;

    MAARALCMLG LVLALLSSSS AEEYVGLSAN QCAVPAKDRV DCGYPHVTPK

    ECNNRGCCFD SRIPGVPWCF KPLQEAECTF




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