Human ARSA ELISA Kit

Alternative name
Human ASA ELISA Kit;Human cerebroside-sulfatase ELISA Kit;Human MLD ELISA Kit;Human arylsulfatase A ELISA Kit;
Catalog
E003765
species
Human
GeneARSA
Standard Curve
Other Species Mouse Arsa ELISA Kit Bovine ARSA ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Arylsulfatase A (ARSA). No significant cross-reactivity or interference between Arylsulfatase A (ARSA) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.056ng/mL.
Intended UseHuman ARSA ELISA Kit allows for the in vitro quantitative determination of ARSA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase A (ARSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase A (ARSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase A (ARSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylsulfatase A (ARSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Arylsulfatase A component C Protein information
Uniprot ID ARSA_HUMAN
Uniprot AC P15289; B2RCA6; B7XD04; F8WCC8; Q6ICI5; Q96CJ0;
UniGene Hs.731715; Hs.88251;
GeneID 410
KEGG hsa:410;
Human Arylsulfatase A component C Protein SEQUENCE
SEQUENCE 507 AA; 53588 MW; 3DDBE1378B4176A6 CRC64;
MGAPRSLLLA LAAGLAVARP PNIVLIFADD LGYGDLGCYG HPSSTTPNLD
QLAAGGLRFT DFYVPVSLCT PSRAALLTGR LPVRMGMYPG VLVPSSRGGL
PLEEVTVAEV LAARGYLTGM AGKWHLGVGP EGAFLPPHQG FHRFLGIPYS
HDQGPCQNLT CFPPATPCDG GCDQGLVPIP LLANLSVEAQ PPWLPGLEAR
YMAFAHDLMA DAQRQDRPFF LYYASHHTHY PQFSGQSFAE RSGRGPFGDS
LMELDAAVGT LMTAIGDLGL LEETLVIFTA DNGPETMRMS RGGCSGLLRC
GKGTTYEGGV REPALAFWPG HIAPGVTHEL ASSLDLLPTL AALAGAPLPN
VTLDGFDLSP LLLGTGKSPR QSLFFYPSYP DEVRGVFAVR TGKYKAHFFT
QGSAHSDTTA DPACHASSSL TAHEPPLLYD LSKDPGENYN LLGGVAGATP
EVLQALKQLQ LLKAQLDAAV TFGPSQVARG EDPALQICCH PGCTPRPACC
HCPDPHA
UCSC uc003bmz.6; human. [P15289-1];

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Category

Human ARSA ELISA Kit

Human ASA ELISA Kit;Human cerebroside-sulfatase ELISA Kit;Human MLD ELISA Kit;Human arylsulfatase A ELISA Kit;