Human ARSA ELISA Kit

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  • Alternative name

    Human ASA ELISA Kit;Human cerebroside-sulfatase ELISA Kit;Human MLD ELISA Kit;Human arylsulfatase A ELISA Kit;

  • Catalog
    E003765
  • species
    Human
  • GeneARSA
  • Standard CurveHuman ARSA ELISA Kit
  • Other Species Mouse Arsa ELISA KitBovine ARSA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Arylsulfatase A (ARSA). No significant cross-reactivity or interference between Arylsulfatase A (ARSA) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.056ng/mL.
  • Intended UseHuman ARSA ELISA Kit allows for the in vitro quantitative determination of ARSA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arylsulfatase A (ARSA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Arylsulfatase A (ARSA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arylsulfatase A (ARSA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Arylsulfatase A (ARSA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Arylsulfatase A component C Protein information
  • Uniprot ID ARSA_HUMAN
  • Uniprot AC P15289; B2RCA6; B7XD04; F8WCC8; Q6ICI5; Q96CJ0;
  • UniGene Hs.731715; Hs.88251;
  • GeneID 410
  • KEGG hsa:410;
  • Human Arylsulfatase A component C Protein SEQUENCE
  • SEQUENCE 507 AA; 53588 MW; 3DDBE1378B4176A6 CRC64;

    MGAPRSLLLA LAAGLAVARP PNIVLIFADD LGYGDLGCYG HPSSTTPNLD

    QLAAGGLRFT DFYVPVSLCT PSRAALLTGR LPVRMGMYPG VLVPSSRGGL

    PLEEVTVAEV LAARGYLTGM AGKWHLGVGP EGAFLPPHQG FHRFLGIPYS

    HDQGPCQNLT CFPPATPCDG GCDQGLVPIP LLANLSVEAQ PPWLPGLEAR

    YMAFAHDLMA DAQRQDRPFF LYYASHHTHY PQFSGQSFAE RSGRGPFGDS

    LMELDAAVGT LMTAIGDLGL LEETLVIFTA DNGPETMRMS RGGCSGLLRC

    GKGTTYEGGV REPALAFWPG HIAPGVTHEL ASSLDLLPTL AALAGAPLPN

    VTLDGFDLSP LLLGTGKSPR QSLFFYPSYP DEVRGVFAVR TGKYKAHFFT

    QGSAHSDTTA DPACHASSSL TAHEPPLLYD LSKDPGENYN LLGGVAGATP

    EVLQALKQLQ LLKAQLDAAV TFGPSQVARG EDPALQICCH PGCTPRPACC

    HCPDPHA

  • UCSC uc003bmz.6; human. [P15289-1];


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