SpecificityThis assay has high sensitivity and excellent specificity for detection of TRACP5alpha. No significant cross-reactivity or interference between TRACP5alpha and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TRACP5alpha and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseHuman TRAP 5a ELISA Kit allows for the in vitro quantitative determination of TRAP 5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the assay: TRACP5alpha ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TRACP5alpha antibody and an TRACP5alpha-HRP conjugate. The assay sample and buffer are incubated together with TRACP5alpha-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TRACP5alpha concentration since TRACP5alpha from samples and TRACP5alpha-HRP conjugate compete for the anti-TRACP5alpha antibody binding site. Since the number of sites is limited, as more sites are occupied by TRACP5alpha from the sample, fewer sites are left to bind TRACP5alpha-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TRACP5alpha concentration in each sample is interpolated from this standard curve.