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Human SDC4 ELISA Kit

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  • Alternative name

    Human SYND4 ELISA Kit;Human amphiglycan ELISA Kit;Human ryudocan core protein ELISA Kit;Human syndecan 4 ELISA Kit;Human syndecan-4 ELISA Kit;Human ryudocan amphiglycan ELISA Kit;Human syndecan 4 (amphiglycan, ryudocan) ELISA Kit;Human syndecan proteoglycan 4 ELISA Kit;
  • Catalog
    E037173
  • species
    Human
  • GeneSDC4
  • SpecificityThe Human Syndecan 4 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Syndecan 4 proteins within the range of 62.5 pg/ml - 4000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman SDC4 ELISA Kit allows for the in vitro quantitative determination of SDC4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Human Syndecan 4 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Syndecan 4 in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Syndecan 4 coated on a 96-well plate. Standards and samples are pipetted into the wells and Syndecan 4 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Syndecan 4 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Syndecan 4 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Syndecan-4 (SDC4) is a protein that in humans is encoded by the SDC4 gene. The protein is found as a homodimer and is a member of the syndecan proteoglycan family. It is mapped to 20q13.12. Syndecan-4 is one of the four vertebrate syndecans and has a molecular weight of ~20 kDa. It is a transmembrane (type I) heparan sulfate proteoglycan that functions as a receptor in intracellular signaling. Syndecan-4 also regulates the actin cytoskeleton, cell adhesion, and cell migration. In addition, Syndecan-4 can activate protein kinase C (PKC). The variable domain of syndecan-4 could be a site of self-association. What's more, Syndecan-4 also binds to phosphatidylinositol (4,5)-bisphosphate (PIP2) through the variable domain and increases PKC activity ten-fold.
  • Human Syndecan-4 Protein information
  • Uniprot ID SDC4_HUMAN
  • Uniprot AC P31431; O00773; Q16833; Q53FN9; Q6FGN3;
  • UniGene Hs.632267;
  • GeneID 6385
  • KEGG hsa:6385;
  • Human Syndecan-4 Protein SEQUENCE

  • SEQUENCE 198 AA; 21642 MW; 8229AA2733F77A10 CRC64;

    MAPARLFALL LFFVGGVAES IRETEVIDPQ DLLEGRYFSG ALPDDEDVVG

    PGQESDDFEL SGSGDLDDLE DSMIGPEVVH PLVPLDNHIP ERAGSGSQVP

    TEPKKLEENE VIPKRISPVE ESEDVSNKVS MSSTVQGSNI FERTEVLAAL

    IVGGIVGILF AVFLILLLMY RMKKKDEGSY DLGKKPIYKK APTNEFYA

  • UCSC uc002xnu.4; human. [P31431-1];



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