Human AIPL1 ELISA Kit

Alternative name
Human AIPL2 ELISA Kit;Human LCA4 ELISA Kit;Human aryl hydrocarbon receptor interacting protein like 1 ELISA Kit;Human aryl-hydrocarbon-interacting protein-like 1 ELISA Kit;
Catalog
E003716
species
Human
GeneAIPL1
Standard Curve
Other Species Mouse Aipl1 ELISA Kit Bovine AIPL1 ELISA Kit Rat Aipl1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of AIPL1. No significant cross-reactivity or interference between AIPL1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AIPL1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman AIPL1 ELISA Kit allows for the in vitro quantitative determination of AIPL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyNeurobiology
Product Description
specificalPrinciple of the assay: AIPL1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AIPL1 antibody and an AIPL1-HRP conjugate. The assay sample and buffer are incubated together with AIPL1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AIPL1 concentration since AIPL1 from samples and AIPL1-HRP conjugate compete for the anti-AIPL1 antibody binding site. Since the number of sites is limited, as more sites are occupied by AIPL1 from the sample, fewer sites are left to bind AIPL1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AIPL1 concentration in each sample is interpolated from this standard curve.
Human Aryl-hydrocarbon-interacting protein-like 1 Protein information
Uniprot ID AIPL1_HUMAN
Uniprot AC Q9NZN9; D3DTM4; Q659W3; Q659W4; Q6ZZB6; Q8N6A0; Q9H873; Q9NS10;
UniGene Hs.279887;
GeneID 23746
KEGG hsa:23746;
Human Aryl-hydrocarbon-interacting protein-like 1 Protein SEQUENCE
SEQUENCE 384 AA; 43903 MW; 47F681A1DC91A82D CRC64;
MDAALLLNVE GVKKTILHGG TGELPNFITG SRVIFHFRTM KCDEERTVID
DSRQVGQPMH IIIGNMFKLE VWEILLTSMR VHEVAEFWCD TIHTGVYPIL
SRSLRQMAQG KDPTEWHVHT CGLANMFAYH TLGYEDLDEL QKEPQPLVFV
IELLQVDAPS DYQRETWNLS NHEKMKAVPV LHGEGNRLFK LGRYEEASSK
YQEAIICLRN LQTKEKPWEV QWLKLEKMIN TLILNYCQCL LKKEEYYEVL
EHTSDILRHH PGIVKAYYVR ARAHAEVWNE AEAKADLQKV LELEPSMQKA
VRRELRLLEN RMAEKQEEER LRCRNMLSQG ATQPPAEPPT EPPAQSSTEP
PAEPPTAPSA ELSAGPPAEP ATEPPPSPGH SLQH
UCSC uc002gcp.5; human. [Q9NZN9-1];

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Human AIPL1 ELISA Kit

Human AIPL2 ELISA Kit;Human LCA4 ELISA Kit;Human aryl hydrocarbon receptor interacting protein like 1 ELISA Kit;Human aryl-hydrocarbon-interacting protein-like 1 ELISA Kit;