Human SYT1 ELISA Kit

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  • Alternative name

    Human synaptotagmin I ELISA Kit;Human sytI ELISA Kit;Human P65 ELISA Kit;Human SVP65 ELISA Kit;Human SYT ELISA Kit;Human synaptotagmin 1 ELISA Kit;Human synaptotagmin-1 ELISA Kit;

  • Catalog
    E037120
  • species
    Human
  • GeneSYT1
  • Standard CurveHuman SYT1 ELISA Kit
  • Other Species Mouse Syt1 ELISA KitBovine SYT1 ELISA KitChicken SYT1 ELISA KitMonkey SYT1 ELISA KitRat Syt1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SYT1. No significant cross-reactivity or interference between SYT1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SYT1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman SYT1 ELISA Kit allows for the in vitro quantitative determination of SYT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This SYT1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human SYT1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||SYT1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-SYT1 antibody and an SYT1-HRP conjugate. The assay sample and buffer are incubated together with SYT1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SYT1 concentration since SYT1 from samples and SYT1-HRP conjugate compete for the anti-SYT1 antibody binding site. Since the number of sites is limited, as more sites are occupied by SYT1 from the sample, fewer sites are left to bind SYT1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SYT1 concentration in each sample is interpolated from this standard curve.
  • Human Synaptotagmin-1 Protein information
  • Uniprot ID SYT1_HUMAN
  • Uniprot AC P21579; Q6AI31;
  • UniGene Hs.310545;
  • GeneID 6857
  • KEGG hsa:6857;
  • Human Synaptotagmin-1 Protein SEQUENCE
  • SEQUENCE 422 AA; 47573 MW; 467F7C58E411AFA9 CRC64;

    MVSESHHEAL AAPPVTTVAT VLPSNATEPA SPGEGKEDAF SKLKEKFMNE

    LHKIPLPPWA LIAIAIVAVL LVLTCCFCIC KKCLFKKKNK KKGKEKGGKN

    AINMKDVKDL GKTMKDQALK DDDAETGLTD GEEKEEPKEE EKLGKLQYSL

    DYDFQNNQLL VGIIQAAELP ALDMGGTSDP YVKVFLLPDK KKKFETKVHR

    KTLNPVFNEQ FTFKVPYSEL GGKTLVMAVY DFDRFSKHDI IGEFKVPMNT

    VDFGHVTEEW RDLQSAEKEE QEKLGDICFS LRYVPTAGKL TVVILEAKNL

    KKMDVGGLSD PYVKIHLMQN GKRLKKKKTT IKKNTLNPYY NESFSFEVPF

    EQIQKVQVVV TVLDYDKIGK NDAIGKVFVG YNSTGAELRH WSDMLANPRR

    PIAQWHTLQV EEEVDAMLAV KK

  • UCSC uc001sys.4; human.;


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