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Human SFTPD ELISA Kit

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  • Alternative name

    Human PSP-D ELISA Kit;Human SP-D ELISA Kit;Human collectin-7 ELISA Kit;Human lung surfactant protein D ELISA Kit;Human COLEC7 ELISA Kit;Human PSPD ELISA Kit;Human SFTP4 ELISA Kit;Human surfactant protein D ELISA Kit;Human pulmonary surfactant-associated protein D ELISA Kit;Human pulmonary surfactant apoprotein ELISA Kit;Human surfactant-associated protein, pulmonary 4 ELISA Kit;
  • Catalog
    E037012
  • species
    Human
  • GeneSFTPD
  • Other Species Mouse Sftpd ELISA KitBovine SFTPD ELISA KitPorcine SFTPD ELISA KitRat Sftpd ELISA KitHuman SFTPC ELISA Kit
  • SpecificityThe Human Surfactant Protein D ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Surfactant Protein D proteins within the range of 625 pg/ml - 40000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity20 pg/ml.
  • Intended UseHuman SFTPD ELISA Kit allows for the in vitro quantitative determination of SFTPD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay||The Human Surfactant Protein D ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Surfactant Protein D in Cell Culture Supernatants, Serum. This assay employs an antibody specific for Human Surfactant Protein D coated on a 96-well plate. Standards and samples are pipetted into the wells and Surfactant Protein D present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Surfactant Protein D antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Surfactant Protein D bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. By fluorescence in situ hybridization, the SP-D gene was localized in 10q22.2-q23.1. On the basis of homology with other collectins, potential functions for SP-D include roles in innate immunity and surfactant metabolism, SP-D is produced in the bronchiolar and terminal epithelium of human fetal lung from about 21 weeks of gestation. What's more, SP-A and SP-D act as dual-function surveillance molecules that reverse orientation and function and become initiators of host-defense reactions.
  • Human Pulmonary surfactant-associated protein D Protein information
  • Uniprot ID SFTPD_HUMAN
  • Uniprot AC P35247; Q5T0M3; Q6FH08; Q86YK9; Q8TCD8; Q9UCJ2; Q9UCJ3;
  • UniGene Hs.253495;
  • GeneID 6441
  • KEGG hsa:6441;
  • Human Pulmonary surfactant-associated protein D Protein SEQUENCE

  • SEQUENCE 375 AA; 37728 MW; 298917699FC40F6A CRC64;

    MLLFLLSALV LLTQPLGYLE AEMKTYSHRT MPSACTLVMC SSVESGLPGR

    DGRDGREGPR GEKGDPGLPG AAGQAGMPGQ AGPVGPKGDN GSVGEPGPKG

    DTGPSGPPGP PGVPGPAGRE GPLGKQGNIG PQGKPGPKGE AGPKGEVGAP

    GMQGSAGARG LAGPKGERGV PGERGVPGNT GAAGSAGAMG PQGSPGARGP

    PGLKGDKGIP GDKGAKGESG LPDVASLRQQ VEALQGQVQH LQAAFSQYKK

    VELFPNGQSV GEKIFKTAGF VKPFTEAQLL CTQAGGQLAS PRSAAENAAL

    QQLVVAKNEA AFLSMTDSKT EGKFTYPTGE SLVYSNWAPG EPNDDGGSED

    CVEIFTNGKW NDRACGEKRL VVCEF

  • UCSC uc001kbh.4; human.;


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