Human UBE2I ELISA Kit

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  • Alternative name

    Human RING-type E3 SUMO transferase UBC9 ELISA Kit;Human SUMO-protein ligase ELISA Kit;Human ubiquitin carrier protein 9 ELISA Kit;Human ubiquitin carrier protein I ELISA Kit;Human Ubiquitin-conjugating enzyme E2 I ELISA Kit;Human ubiquitin-protein ligase I ELISA Kit;Human P18 ELISA Kit;Human UBC9 ELISA Kit;Human UBCE9 ELISA Kit;Human C358B7.1 ELISA Kit;Human ubiquitin conjugating enzyme E2 I ELISA Kit;Human SUMO-conjugating enzyme UBC9 ELISA Kit;Human SUMO-1-protein ligase ELISA Kit;Human ubiquitin conjugating enzyme 9 ELISA Kit;Human ubiquitin conjugating enzyme E2I ELISA Kit;Human ubiquitin-conjugating enzyme E2I (UBC9 homolog, yeast) ELISA Kit;Human ubiquitin-conjugating enzyme E2I (homologous to yeast UBC9) ELISA Kit;Human ubiquitin-conjugating enzyme UbcE2A ELISA Kit;Human ubiquitin-like protein SUMO-1 conjugating enzyme ELISA Kit;Human ubiquitin-protein ligase E2I ELISA Kit;

  • Catalog
    E036910
  • species
    Human
  • GeneUBE2I
  • Standard CurveHuman UBE2I ELISA Kit
  • Other Species Mouse Ube2i ELISA KitChicken UBE2I ELISA KitRat Ube2i ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of UBE2I. No significant cross-reactivity or interference between UBE2I and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between UBE2I and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman UBE2I ELISA Kit allows for the in vitro quantitative determination of UBE2I , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: UBE2I ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-UBE2I antibody and an UBE2I-HRP conjugate. The assay sample and buffer are incubated together with UBE2I-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the UBE2I concentration since UBE2I from samples and UBE2I-HRP conjugate compete for the anti-UBE2I antibody binding site. Since the number of sites is limited, as more sites are occupied by UBE2I from the sample, fewer sites are left to bind UBE2I-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The UBE2I concentration in each sample is interpolated from this standard curve.
  • Human SUMO-conjugating enzyme UBC9 Protein information
  • Uniprot ID UBC9_HUMAN
  • Uniprot AC P63279; D3DU69; P50550; Q15698; Q59GX1; Q86VB3;
  • UniGene Hs.302903;
  • GeneID 7329
  • KEGG hsa:7329;
  • Human SUMO-conjugating enzyme UBC9 Protein SEQUENCE
  • SEQUENCE 158 AA; 18007 MW; E2C826E9C8D0683D CRC64;

    MSGIALSRLA QERKAWRKDH PFGFVAVPTK NPDGTMNLMN WECAIPGKKG

    TPWEGGLFKL RMLFKDDYPS SPPKCKFEPP LFHPNVYPSG TVCLSILEED

    KDWRPAITIK QILLGIQELL NEPNIQDPAQ AEAYTIYCQN RVEYEKRVRA

    QAKKFAPS

  • UCSC uc002clc.2; human.;



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