Human SRXN1 ELISA Kit

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  • Alternative name

    Human C20orf139 ELISA Kit;Human SRX ELISA Kit;Human Npn3 ELISA Kit;Human SRX1 ELISA Kit;Human sulfiredoxin 1 ELISA Kit;Human sulfiredoxin-1 ELISA Kit;

  • Catalog
    E036894
  • species
    Human
  • GeneSRXN1
  • Standard CurveHuman SRXN1 ELISA Kit
  • Other Species Mouse Srxn1 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural HumanSulfiredoxin-1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman SRXN1 ELISA Kit allows for the in vitro quantitative determination of SRXN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of humanSulfiredoxin-1concentrations in serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids. Principle of the Assay||The ELISA is based on the competitive binding enzyme immunoassaytechnique. The microtiter plate provided in this kit has been pre-coated with an antibody specific toSulfiredoxin-1, During the reaction,Sulfiredoxin-1in the sample or standard competes with a fixed amount of biotin-labeledSulfiredoxin-1 for sites on a pre-coated Monoclonal antibody specific to Sulfiredoxin-1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Thena TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sulfiredoxin-1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sulfiredoxin-1 Protein information
  • Uniprot ID SRXN1_HUMAN
  • Uniprot AC Q9BYN0; B2R543; Q8NDM3; Q96AK6;
  • UniGene Hs.516830;
  • GeneID 140809
  • KEGG hsa:140809;
  • Human Sulfiredoxin-1 Protein SEQUENCE
  • SEQUENCE 137 AA; 14259 MW; 44F2C4256E5574D0 CRC64;

    MGLRAGGTLG RAGAGRGAPE GPGPSGGAQG GSIHSGRIAA VHNVPLSVLI

    RPLPSVLDPA KVQSLVDTIR EDPDSVPPID VLWIKGAQGG DYFYSFGGCH

    RYAAYQQLQR ETIPAKLVQS TLSDLRVYLG ASTPDLQ

  • UCSC uc002wea.5; human.;


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