Human DDC ELISA Kit

Alternative name
Human AADC ELISA Kit;Human dopa decarboxylase ELISA Kit;Human DDC ELISA Kit;Human aromatic-L-amino-acid decarboxylase ELISA Kit;Human dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit;
Catalog
E003684
species
Human
GeneDDC
Standard Curve
Other Species Mouse Ddc ELISA Kit Bovine DDC ELISA Kit Guinea Pig DDC ELISA Kit Porcine DDC ELISA Kit Rat Ddc ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of DDC. No significant cross-reactivity or interference between DDC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDC and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman DDC ELISA Kit allows for the in vitro quantitative determination of DDC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: DDC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDC antibody and an DDC-HRP conjugate. The assay sample and buffer are incubated together with DDC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDC concentration since DDC from samples and DDC-HRP conjugate compete for the anti-DDC antibody binding site. Since the number of sites is limited, as more sites are occupied by DDC from the sample, fewer sites are left to bind DDC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDC concentration in each sample is interpolated from this standard curve.
Human Aromatic-L-amino-acid decarboxylase Protein information
Uniprot ID DDC_HUMAN
Uniprot AC P20711; C9IYA0; E7ER62; E7EU95; Q16723; Q5W5T9; Q75MJ6;
UniGene Hs.359698;
GeneID 1644
KEGG hsa:1644;
Human Aromatic-L-amino-acid decarboxylase Protein SEQUENCE
SEQUENCE 480 AA; 53926 MW; 0A2CFF9A39BDE298 CRC64;
MNASEFRRRG KEMVDYMANY MEGIEGRQVY PDVEPGYLRP LIPAAAPQEP
DTFEDIINDV EKIIMPGVTH WHSPYFFAYF PTASSYPAML ADMLCGAIGC
IGFSWAASPA CTELETVMMD WLGKMLELPK AFLNEKAGEG GGVIQGSASE
ATLVALLAAR TKVIHRLQAA SPELTQAAIM EKLVAYSSDQ AHSSVERAGL
IGGVKLKAIP SDGNFAMRAS ALQEALERDK AAGLIPFFMV ATLGTTTCCS
FDNLLEVGPI CNKEDIWLHV DAAYAGSAFI CPEFRHLLNG VEFADSFNFN
PHKWLLVNFD CSAMWVKKRT DLTGAFRLDP TYLKHSHQDS GLITDYRHWQ
IPLGRRFRSL KMWFVFRMYG VKGLQAYIRK HVQLSHEFES LVRQDPRFEI
CVEVILGLVC FRLKGSNKVN EALLQRINSA KKIHLVPCHL RDKFVLRFAI
CSRTVESAHV QRAWEHIKEL AADVLRAERE
UCSC uc003tpf.5; human. [P20711-1];

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Category

Human DDC ELISA Kit

Human AADC ELISA Kit;Human dopa decarboxylase ELISA Kit;Human DDC ELISA Kit;Human aromatic-L-amino-acid decarboxylase ELISA Kit;Human dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit;