Human DDC ELISA Kit

Contact Us
  • Alternative name

    Human AADC ELISA Kit;Human dopa decarboxylase ELISA Kit;Human DDC ELISA Kit;Human aromatic-L-amino-acid decarboxylase ELISA Kit;Human dopa decarboxylase (aromatic L-amino acid decarboxylase) ELISA Kit;

  • Catalog
    E003684
  • species
    Human
  • GeneDDC
  • Standard CurveHuman DDC ELISA Kit
  • Other Species Mouse Ddc ELISA KitBovine DDC ELISA KitGuinea Pig DDC ELISA KitPorcine DDC ELISA KitRat Ddc ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DDC. No significant cross-reactivity or interference between DDC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDC and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman DDC ELISA Kit allows for the in vitro quantitative determination of DDC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: DDC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDC antibody and an DDC-HRP conjugate. The assay sample and buffer are incubated together with DDC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDC concentration since DDC from samples and DDC-HRP conjugate compete for the anti-DDC antibody binding site. Since the number of sites is limited, as more sites are occupied by DDC from the sample, fewer sites are left to bind DDC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDC concentration in each sample is interpolated from this standard curve.
  • Human Aromatic-L-amino-acid decarboxylase Protein information
  • Uniprot ID DDC_HUMAN
  • Uniprot AC P20711; C9IYA0; E7ER62; E7EU95; Q16723; Q5W5T9; Q75MJ6;
  • UniGene Hs.359698;
  • GeneID 1644
  • KEGG hsa:1644;
  • Human Aromatic-L-amino-acid decarboxylase Protein SEQUENCE
  • SEQUENCE 480 AA; 53926 MW; 0A2CFF9A39BDE298 CRC64;

    MNASEFRRRG KEMVDYMANY MEGIEGRQVY PDVEPGYLRP LIPAAAPQEP

    DTFEDIINDV EKIIMPGVTH WHSPYFFAYF PTASSYPAML ADMLCGAIGC

    IGFSWAASPA CTELETVMMD WLGKMLELPK AFLNEKAGEG GGVIQGSASE

    ATLVALLAAR TKVIHRLQAA SPELTQAAIM EKLVAYSSDQ AHSSVERAGL

    IGGVKLKAIP SDGNFAMRAS ALQEALERDK AAGLIPFFMV ATLGTTTCCS

    FDNLLEVGPI CNKEDIWLHV DAAYAGSAFI CPEFRHLLNG VEFADSFNFN

    PHKWLLVNFD CSAMWVKKRT DLTGAFRLDP TYLKHSHQDS GLITDYRHWQ

    IPLGRRFRSL KMWFVFRMYG VKGLQAYIRK HVQLSHEFES LVRQDPRFEI

    CVEVILGLVC FRLKGSNKVN EALLQRINSA KKIHLVPCHL RDKFVLRFAI

    CSRTVESAHV QRAWEHIKEL AADVLRAERE

  • UCSC uc003tpf.5; human. [P20711-1];


Related products with Human DDC ELISA Kit


tag

Inquiry About Human DDC ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!