Human SDF4 ELISA Kit

Alternative name
Human Cab45 ELISA Kit;Human Stromal cell-derived factor 4 ELISA Kit;Human SDF-4 ELISA Kit;Human stromal cell derived factor 4 ELISA Kit;Human 45 kDa calcium-binding protein ELISA Kit;
Catalog
E036807
species
Human
GeneSDF4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman SDF4 ELISA Kit allows for the in vitro quantitative determination of SDF4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: SDF4 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for SDF4. Standards or samples are then added to the microtiter plate wells and SDF4 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of SDF4 present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for SDF4 are added to each well to "sandwich" the SDF4 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain SDF4 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SDF4 concentration in each sample is interpolated from this standard curve.
Human 45 kDa calcium-binding protein Protein information
Uniprot ID CAB45_HUMAN
Uniprot AC Q96AA1; Q9NZP7; Q9UN53;
UniGene Hs.42806;
GeneID 51150
KEGG hsa:51150;
Human 45 kDa calcium-binding protein Protein SEQUENCE
SEQUENCE 362 AA; 41807 MW; 440C6990149AAE2C CRC64;
MVWPWVAMAS RWGPLIGLAP CCLWLLGAVL LMDASARPAN HSSTRERVAN
REENEILPPD HLNGVKLEMD GHLNRGFHQE VFLGKDLGGF DEDAEPRRSR
RKLMVIFSKV DVNTDRKISA KEMQRWIMEK TAEHFQEAME ESKTHFRAVD
PDGDGHVSWD EYKVKFLASK GHSEKEVADA IRLNEELKVD EETQEVLENL
KDRWYQADSP PADLLLTEEE FLSFLHPEHS RGMLRFMVKE IVRDLDQDGD
KQLSVPEFIS LPVGTVENQQ GQDIDDNWVK DRKKEFEELI DSNHDGIVTA
EELESYMDPM NEYNALNEAK QMIAVADENQ NHHLEPEEVL KYSEFFTGSK
LVDYARSVHE EF
UCSC uc001adh.5; human. [Q9BRK5-1];

Category

Human SDF4 ELISA Kit

Human Cab45 ELISA Kit;Human Stromal cell-derived factor 4 ELISA Kit;Human SDF-4 ELISA Kit;Human stromal cell derived factor 4 ELISA Kit;Human 45 kDa calcium-binding protein ELISA Kit;