Human SFN ELISA Kit

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  • Alternative name

    Human epithelial cell marker protein 1 ELISA Kit;Human stratifin ELISA Kit;Human HME1 ELISA Kit;Human YWHAS ELISA Kit;Human 14-3-3 protein sigma ELISA Kit;Human 14-3-3 sigma ELISA Kit;

  • Catalog
    E036770
  • species
    Human
  • GeneSFN
  • Other Species Mouse Sfn ELISA KitBovine SFN ELISA KitSheep SFN ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SFN. No significant cross-reactivity or interference between human SFN and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SFN is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
  • Intended UseHuman SFN ELISA Kit allows for the in vitro quantitative determination of SFN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFN bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human 14-3-3 protein sigma Protein information
  • Uniprot ID 1433S_HUMAN
  • Uniprot AC P31947; Q6FH30; Q6FH51; Q96DH0;
  • UniGene Hs.523718;
  • GeneID 2810
  • KEGG hsa:2810;
  • Human 14-3-3 protein sigma Protein SEQUENCE
  • SEQUENCE 248 AA; 27774 MW; 7F4B44E3AA59ECE6 CRC64;

    MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN

    VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL

    GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA

    YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE

    AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS

  • UCSC uc001bnc.2; human. [P31947-1];



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