Human SFN ELISA Kit

Alternative name
Human epithelial cell marker protein 1 ELISA Kit;Human stratifin ELISA Kit;Human HME1 ELISA Kit;Human YWHAS ELISA Kit;Human 14-3-3 protein sigma ELISA Kit;Human 14-3-3 sigma ELISA Kit;
Catalog
E036770
species
Human
GeneSFN
SpecificityThis assay has high sensitivity and excellent specificity for detection of human SFN. No significant cross-reactivity or interference between human SFN and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of human SFN is typically less than 0.195 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
Intended UseHuman SFN ELISA Kit allows for the in vitro quantitative determination of SFN , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SFN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SFN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SFN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SFN bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human 14-3-3 protein sigma Protein information
Uniprot ID 1433S_HUMAN
Uniprot AC P31947; Q6FH30; Q6FH51; Q96DH0;
UniGene Hs.523718;
GeneID 2810
KEGG hsa:2810;
Human 14-3-3 protein sigma Protein SEQUENCE
SEQUENCE 248 AA; 27774 MW; 7F4B44E3AA59ECE6 CRC64;
MERASLIQKA KLAEQAERYE DMAAFMKGAV EKGEELSCEE RNLLSVAYKN
VVGGQRAAWR VLSSIEQKSN EEGSEEKGPE VREYREKVET ELQGVCDTVL
GLLDSHLIKE AGDAESRVFY LKMKGDYYRY LAEVATGDDK KRIIDSARSA
YQEAMDISKK EMPPTNPIRL GLALNFSVFH YEIANSPEEA ISLAKTTFDE
AMADLHTLSE DSYKDSTLIM QLLRDNLTLW TADNAGEEGG EAPQEPQS
UCSC uc001bnc.2; human. [P31947-1];

Category

Human SFN ELISA Kit

Human epithelial cell marker protein 1 ELISA Kit;Human stratifin ELISA Kit;Human HME1 ELISA Kit;Human YWHAS ELISA Kit;Human 14-3-3 protein sigma ELISA Kit;Human 14-3-3 sigma ELISA Kit;