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Alternative name
Steroid 5-alpha reductase ELISA KIT; Steroid 5-alpha reductasesr5a ELISA KIT;
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Catalog
E036711
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species
Human
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GeneSRD5a
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SpecificityThis assay has high sensitivity and excellent specificity for detection of SRD5alphaR. No significant cross-reactivity or interference between SRD5alphaR and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SRD5alphaR and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.1 ng/mL.
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Intended UseHuman SRD5a ELISA Kit allows for the in vitro quantitative determination of SRD5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: SRD5alphaR ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SRD5alphaR antibody and an SRD5alphaR-HRP conjugate. The assay sample and buffer are incubated together with SRD5alphaR-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SRD5alphaR concentration since SRD5alphaR from samples and SRD5alphaR-HRP conjugate compete for the anti-SRD5alphaR antibody binding site. Since the number of sites is limited, as more sites are occupied by SRD5alphaR from the sample, fewer sites are left to bind SRD5alphaR-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SRD5alphaR concentration in each sample is interpolated from this standard curve.
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