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Alternative name
P-selectin glycoprotein ligand 1 ELISA KIT; Selectin P ligand ELISA KIT; CD_antigen: CD162SELPLG ELISA KIT; PSGL-1 ELISA KIT
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Catalog
E036538
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species
Human
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GenesPSGL-1
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SpecificityThe interference of circulating factors of the immune system was evaluated by spiking these proteins at physiologically relevant concentrations into a human PSGL-1 positive serum. There was no cross-reactivity detected.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman sPSGL-1 ELISA Kit allows for the in vitro quantitative determination of sPSGL-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyImmunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
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Product Description
specificalPrinciple of the Assay: An anti-human PSGL-1 coating antibody is adsorbed onto microwells. Human PSGL-1 present in the unknown or calibrator binds to antibodies adsorbed to the microwells. A biotin-conjugated anti-human PSGL-1 antibody is added and binds to human PSGL-1 captured by the first antibody. Following incubation unbound biotin-conjugated anti-human PSGL-1 antibody is removed during a wash step. Streptavidin-HRP is added and binds to the biotin-conjugated anti-human PSGL-1 antibody. Following incubation unbound Streptavidin-HRP is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A colored product is formed in proportion to the amount of human PSGL-1 present in the unknown or calibrator. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A calibration curve is prepared from 6 human PSGL-1 calibrator dilutions and human PSGL-1 unknown concentration determined.
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