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Human SPI1 ELISA Kit

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  • Alternative name

    Human 31 kDa-transforming protein ELISA Kit;Human OF ELISA Kit;Human PU.1 ELISA Kit;Human SFPI1 ELISA Kit;Human SPI-1 ELISA Kit;Human SPI-A ELISA Kit;Human Spi-1 proto-oncogene ELISA Kit;Human transcription factor PU.1 ELISA Kit;Human 31 kDa transforming protein ELISA Kit;Human hematopoietic transcription factor PU.1 ELISA Kit;Human spleen focus forming virus (SFFV) proviral integration oncogene spi1 ELISA Kit;
  • Catalog
    E036485
  • species
    Human
  • GeneSPI1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SPI1. No significant cross-reactivity or interference between human SPI1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SPI1 is typically less than 9.4 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
  • Intended UseHuman SPI1 ELISA Kit allows for the in vitro quantitative determination of SPI1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPI1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPI1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPI1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPI1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Transcription factor PU.1 Protein information
  • Uniprot ID SPI1_HUMAN
  • Uniprot AC P17947;
  • UniGene Hs.502511;
  • GeneID 6688
  • KEGG hsa:6688;
  • Human Transcription factor PU.1 Protein SEQUENCE

  • SEQUENCE 270 AA; 31083 MW; 9592DAA1D85053ED CRC64;

    MLQACKMEGF PLVPPPSEDL VPYDTDLYQR QTHEYYPYLS SDGESHSDHY

    WDFHPHHVHS EFESFAENNF TELQSVQPPQ LQQLYRHMEL EQMHVLDTPM

    VPPHPSLGHQ VSYLPRMCLQ YPSLSPAQPS SDEEEGERQS PPLEVSDGEA

    DGLEPGPGLL PGETGSKKKI RLYQFLLDLL RSGDMKDSIW WVDKDKGTFQ

    FSSKHKEALA HRWGIQKGNR KKMTYQKMAR ALRNYGKTGE VKKVKKKLTY

    QFSGEVLGRG GLAERRHPPH

  • UCSC uc001nfb.2; human. [P17947-1];



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