Human SGPP1 ELISA Kit

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  • Alternative name

    Human SPPase1 ELISA Kit;Human spp1 ELISA Kit;Human hSPP1 ELISA Kit;Human hSPPase1 ELISA Kit;Human sphingosine-1-phosphatase 1 ELISA Kit;Human sphingosine-1-phosphate phosphatase 1 ELISA Kit;

  • Catalog
    E036458
  • species
    Human
  • GeneSGPP1
  • Standard CurveHuman SGPP1 ELISA Kit
  • Other Species Mouse Sgpp1 ELISA KitRat Sgpp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). No significant cross-reactivity or interference between Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.062ng/mL.
  • Intended UseHuman SGPP1 ELISA Kit allows for the in vitro quantitative determination of SGPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sphingosine-1-phosphate phosphatase 1 Protein information
  • Uniprot ID SGPP1_HUMAN
  • Uniprot AC Q9BX95; B2RAH0; Q9H189;
  • UniGene Hs.24678;
  • GeneID 81537
  • KEGG hsa:81537;
  • Human Sphingosine-1-phosphate phosphatase 1 Protein SEQUENCE
  • SEQUENCE 441 AA; 49108 MW; 86A018DDDA3A3019 CRC64;

    MSLRQRLAQL VGRLQDPQKV ARFQRLCGVE APPRRSADRR EDEKAEAPLA

    GDPRLRGRQP GAPGGPQPPG SDRNQCPAKP DGGGAPNGVR NGLAAELGPA

    SPRRAGALRR NSLTGEEGQL ARVSNWPLYC LFCFGTELGN ELFYILFFPF

    WIWNLDPLVG RRLVVIWVLV MYLGQCTKDI IRWPRPASPP VVKLEVFYNS

    EYSMPSTHAM SGTAIPISMV LLTYGRWQYP LIYGLILIPC WCSLVCLSRI

    YMGMHSILDI IAGFLYTILI LAVFYPFVDL IDNFNQTHKY APFIIIGLHL

    ALGIFSFTLD TWSTSRGDTA EILGSGAGIA CGSHVTYNMG LVLDPSLDTL

    PLAGPPITVT LFGKAILRIL IGMVFVLIIR DVMKKITIPL ACKIFNIPCD

    DIRKARQHME VELPYRYITY GMVGFSITFF VPYIFFFIGI S

  • UCSC uc001xgj.5; human.;




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