Human SGPP1 ELISA Kit

Alternative name
Human SPPase1 ELISA Kit;Human spp1 ELISA Kit;Human hSPP1 ELISA Kit;Human hSPPase1 ELISA Kit;Human sphingosine-1-phosphatase 1 ELISA Kit;Human sphingosine-1-phosphate phosphatase 1 ELISA Kit;
Catalog
E036458
species
Human
GeneSGPP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). No significant cross-reactivity or interference between Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.062ng/mL.
Intended UseHuman SGPP1 ELISA Kit allows for the in vitro quantitative determination of SGPP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sphingosine-1-Phosphate Phosphatase 1 (SGPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sphingosine-1-Phosphate Phosphatase 1 (SGPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sphingosine-1-Phosphate Phosphatase 1 (SGPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Sphingosine-1-phosphate phosphatase 1 Protein information
Uniprot ID SGPP1_HUMAN
Uniprot AC Q9BX95; B2RAH0; Q9H189;
UniGene Hs.24678;
GeneID 81537
KEGG hsa:81537;
Human Sphingosine-1-phosphate phosphatase 1 Protein SEQUENCE
SEQUENCE 441 AA; 49108 MW; 86A018DDDA3A3019 CRC64;
MSLRQRLAQL VGRLQDPQKV ARFQRLCGVE APPRRSADRR EDEKAEAPLA
GDPRLRGRQP GAPGGPQPPG SDRNQCPAKP DGGGAPNGVR NGLAAELGPA
SPRRAGALRR NSLTGEEGQL ARVSNWPLYC LFCFGTELGN ELFYILFFPF
WIWNLDPLVG RRLVVIWVLV MYLGQCTKDI IRWPRPASPP VVKLEVFYNS
EYSMPSTHAM SGTAIPISMV LLTYGRWQYP LIYGLILIPC WCSLVCLSRI
YMGMHSILDI IAGFLYTILI LAVFYPFVDL IDNFNQTHKY APFIIIGLHL
ALGIFSFTLD TWSTSRGDTA EILGSGAGIA CGSHVTYNMG LVLDPSLDTL
PLAGPPITVT LFGKAILRIL IGMVFVLIIR DVMKKITIPL ACKIFNIPCD
DIRKARQHME VELPYRYITY GMVGFSITFF VPYIFFFIGI S
UCSC uc001xgj.5; human.;

Category

Human SGPP1 ELISA Kit

Human SPPase1 ELISA Kit;Human spp1 ELISA Kit;Human hSPP1 ELISA Kit;Human hSPPase1 ELISA Kit;Human sphingosine-1-phosphatase 1 ELISA Kit;Human sphingosine-1-phosphate phosphatase 1 ELISA Kit;