Human SGPL1 ELISA Kit

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  • Alternative name

    Human S1PL ELISA Kit;Human SP-lyase 1 ELISA Kit;Human SPL 1 ELISA Kit;Human hSPL ELISA Kit;Human sphingosine-1-phosphate aldolase ELISA Kit;Human S1PL ELISA Kit;Human SPL ELISA Kit;Human sphingosine-1-phosphate lyase 1 ELISA Kit;Human SP-lyase 1 ELISA Kit;Human SPL 1 ELISA Kit;

  • Catalog
    E036455
  • species
    Human
  • GeneSGPL1
  • Standard CurveHuman SGPL1 ELISA Kit
  • Other Species Mouse Sgpl1 ELISA KitRat Sgpl1 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural human SPL. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman SGPL1 ELISA Kit allows for the in vitro quantitative determination of SGPL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of human Sphingosine-1-phosphate lyase 1, SPL concentrations in serum, Plasma, tissue homogenates and Cell culture supernates and Other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to SPL. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SPL and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SPL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SPL in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Sphingosine-1-phosphate lyase 1 Protein information
  • Uniprot ID SGPL1_HUMAN
  • Uniprot AC O95470; B2RBD4; Q7Z732; Q9ULG8; Q9UN89;
  • UniGene Hs.499984;
  • GeneID 8879
  • KEGG hsa:8879;
  • Human Sphingosine-1-phosphate lyase 1 Protein SEQUENCE
  • SEQUENCE 568 AA; 63524 MW; 3B16FDEFC4B2FDB6 CRC64;

    MPSTDLLMLK AFEPYLEILE VYSTKAKNYV NGHCTKYEPW QLIAWSVVWT

    LLIVWGYEFV FQPESLWSRF KKKCFKLTRK MPIIGRKIQD KLNKTKDDIS

    KNMSFLKVDK EYVKALPSQG LSSSAVLEKL KEYSSMDAFW QEGRASGTVY

    SGEEKLTELL VKAYGDFAWS NPLHPDIFPG LRKIEAEIVR IACSLFNGGP

    DSCGCVTSGG TESILMACKA YRDLAFEKGI KTPEIVAPQS AHAAFNKAAS

    YFGMKIVRVP LTKMMEVDVR AMRRAISRNT AMLVCSTPQF PHGVIDPVPE

    VAKLAVKYKI PLHVDACLGG FLIVFMEKAG YPLEHPFDFR VKGVTSISAD

    THKYGYAPKG SSLVLYSDKK YRNYQFFVDT DWQGGIYASP TIAGSRPGGI

    SAACWAALMH FGENGYVEAT KQIIKTARFL KSELENIKGI FVFGNPQLSV

    IALGSRDFDI YRLSNLMTAK GWNLNQLQFP PSIHFCITLL HARKRVAIQF

    LKDIRESVTQ IMKNPKAKTT GMGAIYGMAQ TTVDRNMVAE LSSVFLDSLY

    STDTVTQGSQ MNGSPKPH

  • UCSC uc001jrm.4; human.;




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