Human SPH ELISA Kit

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  • Alternative name

    Sphingomyelinase C ELISA KIT; Sphingomyelinase CspH ELISA KIT; BCE_0745 ELISA KIT

  • Catalog
    E036454
  • species
    Human
  • GeneSPH
  • Standard CurveHuman SPH ELISA Kit
  • Other Species Human SM/SPH ELISA KitMouse SPH ELISA KitBovine SPH ELISA KitCanine SPH ELISA KitChicken SPH ELISA KitGeneral SPH ELISA KitPorcine SPH ELISA KitRabbit SPH ELISA KitRat SPH ELISA KitCamel SPH ELISA KitGoat SPH ELISA KitGuinea Pig SPH ELISA KitHamster SPH ELISA KitHorse SPH ELISA KitMonkey SPH ELISA KitSheep SPH ELISA Kit
  • SpecificityNo cross-reaction with other factors.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.06 ng/ml.
  • Intended UseHuman SPH ELISA Kit allows for the in vitro quantitative determination of SPH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Priciple of the assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human SPH monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.


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