Human S1PR5 ELISA Kit

Alternative name
Human S1P receptor 5 ELISA Kit;Human S1P5 ELISA Kit;Human endothelial differentiation G-protein-coupled receptor 8 ELISA Kit;Human sphingosine 1-phosphate receptor Edg-8 ELISA Kit;Human S1P receptor Edg-8 ELISA Kit;Human EDG8 ELISA Kit;Human Edg-8 ELISA Kit;Human SPPR-1 ELISA Kit;Human SPPR-2 ELISA Kit;Human sphingosine-1-phosphate receptor 5 ELISA Kit;Human sphingosine 1-phosphate receptor 5 ELISA Kit;Human endothelial differentiation, sphingolipid G-protein-coupled receptor, 8 ELISA Kit;Human sphingosine 1-phosphate receptor EDG8 ELISA Kit;
Catalog
E036446
species
Human
GeneS1PR5
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<46.9pg/ml
Intended UseHuman S1PR5 ELISA Kit allows for the in vitro quantitative determination of S1PR5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human S1PR5 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human S1PR5 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human S1PR5 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human S1PR5 can be calculated.
Human Sphingosine 1-phosphate receptor 5 Protein information
Uniprot ID S1PR5_HUMAN
Uniprot AC STVLPLYA KAYVLFCVLA FVGILAAICA LYARIYCQVR ANARRLPARP GTAGTTSTRA
UniGene Hs.501561;
GeneID 53637
KEGG hsa:53637;
Human Sphingosine 1-phosphate receptor 5 Protein SEQUENCE
SEQUENCE 398 AA; 41775 MW; 34ADC6C40D8250BF CRC64;
MESGLLRPAP VSEVIVLHYN YTGKLRGARY QPGAGLRADA VVCLAVCAFI
VLENLAVLLV LGRHPRFHAP MFLLLGSLTL SDLLAGAAYA ANILLSGPLT
LKLSPALWFA REGGVFVALT ASVLSLLAIA LERSLTMARR GPAPVSSRGR
TLAMAAAAWG VSLLLGLLPA LGWNCLGRLD ACSTVLPLYA KAYVLFCVLA
FVGILAAICA LYARIYCQVR ANARRLPARP GTAGTTSTRA RRKPRSLALL
RTLSVVLLAF VACWGPLFLL LLLDVACPAR TCPVLLQADP FLGLAMANSL
LNPIIYTLTN RDLRHALLRL VCCGRHSCGR DPSGSQQSAS AAEASGGLRR
CLPPGLDGSF SGSERSSPQR DGLDTSGSTG SPGAPTAART LVSEPAAD
UCSC uc002mot.2; human. [Q9H228-1];

Category

Human S1PR5 ELISA Kit