SpecificityThis assay has high sensitivity and excellent specificity for detection of human SAT1. No significant cross-reactivity or interference between human SAT1 and analogues was observed.
SensitivityThe minimum detectable dose of human SAT1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
Intended UseHuman SAT1 ELISA Kit allows for the in vitro quantitative determination of SAT1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SAT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SAT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SAT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SAT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Diamine acetyltransferase 1 Protein information
Uniprot ID
SAT1_HUMAN
Uniprot AC
P21673;
UniGene
Hs.28491;
GeneID
6303
KEGG
hsa:6303;
Human Diamine acetyltransferase 1 Protein SEQUENCE