Human SPAM1 ELISA Kit

Alternative name
Human hyal-PH20 ELISA Kit;Human hyaluronoglucosaminidase PH-20 ELISA Kit;Human sperm adhesion molecule 1 ELISA Kit;Human sperm surface protein PH-20 ELISA Kit;Human HYAL3 ELISA Kit;Human PH20 ELISA Kit;Human HEL-S-96n ELISA Kit;Human HYA1 ELISA Kit;Human HYAL1 ELISA Kit;Human HYAL5 ELISA Kit;Human PH-20 ELISA Kit;Human SPAG15 ELISA Kit;Human hyaluronidase PH-20 ELISA Kit;Human PH-20 hyaluronidase ELISA Kit;Human epididymis secretory sperm binding protein Li 96n ELISA Kit;Human sperm adhesion molecule 1 (PH-20 hyaluronidase, zona pellucida binding) ELISA Kit;
Catalog
E036348
species
Human
GeneSPAM1
SpecificityThis assay has high sensitivity and excellent specificity for detection of human SPAM1. No significant cross-reactivity or interference between human SPAM1 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity3.9 pg/ml
Intended UseHuman SPAM1 ELISA Kit allows for the in vitro quantitative determination of SPAM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPAM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPAM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPAM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPAM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Human Hyaluronidase PH-20 Protein information
Uniprot ID HYALP_HUMAN
Uniprot AC P38567; Q8TC30;
UniGene Hs.121494;
GeneID 6677
KEGG hsa:6677;
Human Hyaluronidase PH-20 Protein SEQUENCE
SEQUENCE 509 AA; 57848 MW; 5ADB4739747E32E8 CRC64;
MGVLKFKHIF FRSFVKSSGV SQIVFTFLLI PCCLTLNFRA PPVIPNVPFL
WAWNAPSEFC LGKFDEPLDM SLFSFIGSPR INATGQGVTI FYVDRLGYYP
YIDSITGVTV NGGIPQKISL QDHLDKAKKD ITFYMPVDNL GMAVIDWEEW
RPTWARNWKP KDVYKNRSIE LVQQQNVQLS LTEATEKAKQ EFEKAGKDFL
VETIKLGKLL RPNHLWGYYL FPDCYNHHYK KPGYNGSCFN VEIKRNDDLS
WLWNESTALY PSIYLNTQQS PVAATLYVRN RVREAIRVSK IPDAKSPLPV
FAYTRIVFTD QVLKFLSQDE LVYTFGETVA LGASGIVIWG TLSIMRSMKS
CLLLDNYMET ILNPYIINVT LAAKMCSQVL CQEQGVCIRK NWNSSDYLHL
NPDNFAIQLE KGGKFTVRGK PTLEDLEQFS EKFYCSCYST LSCKEKADVK
DTDAVDVCIA DGVCIDAFLK PPMETEEPQI FYNASPSTLS ATMFIVSILF
LIISSVASL
UCSC uc003vle.4; human. [P38567-1];

Category

Human SPAM1 ELISA Kit