Human SPDYE6 ELISA Kit

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  • Catalog
    E036340
  • species
    Human
  • GeneSPDYE6
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human SPDYE6. No significant cross-reactivity or interference between human SPDYE6 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human SPDYE6 is typically less than 5.88 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseHuman SPDYE6 ELISA Kit allows for the in vitro quantitative determination of SPDYE6 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPDYE6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPDYE6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPDYE6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPDYE6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Putative speedy protein E6 Protein information
  • Uniprot ID SPDE6_HUMAN
  • Uniprot AC P0CI01;
  • Human Putative speedy protein E6 Protein SEQUENCE
  • SEQUENCE 402 AA; 48349 MW; 882348A9F0F3CB0B CRC64;

    MDRTETRFRK RGQITGKITT SRQPHPQNEQ SPQRSTSGYP LQEVVDDEML

    GPSAPGVDPS PPCRSLGWKR KREWSDESEE EPEKELAPEP EETWVVEMLC

    GLKMKLKQQR VSSILPEHHK DFNSQLAPGV DPSPPHRSFC WKRKMEWWDE

    SEESLEEEPR KVLAPEPEEI WVAEMLCGLK MKLKRRRVSL VLPEHHEAFN

    RLLEDPVIKR FLAWDKDLRV SDKYLLAMVI AYFSRAGFPS WQYQRIHFFL

    ALYLANDMEE DDEDSKQNIF HFLYRKNRSR IPLLRKRWFQ LGHSMNPRAR

    KNRSRIPLLR KRRFQLYRST NPRARKNRSR IPLLRKRRFQ LYRSMNSRAR

    KNRSQIVLFQ KRRFHFFCSM SCRAWVSPEE LEEIQAYDPE HWVWARDRAH

    LS




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