Intended UseHuman sPECAM-1 ELISA Kit allows for the in vitro quantitative determination of sPECAM-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalIntended Use The human sPECAM-1 ELISA is an enzyme-linked immunosorbent assay for the detection of human sPECAM-1. For research use only, not for use in diagnostic procedures.
PRINCIPLE OF THE TEST An anti-human sPECAM-1 coating antibody is adsorbed onto microwells. Human sPECAM-1 present in the unknown or calibrator binds to antibodies adsorbed to the microwells. A HRP-conjugated anti-human sPECAM-1 antibody is added and binds to human sPECAM-1 captured by the first antibody. Following incubation unbound HRP-conjugated anti-human sPECAM-1 is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human sPECAM-1 present in the unknown or calibrator. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A calibrator curve is prepared from 7 human sPECAM-1 calibrator dilutions and human sPECAM-1 concentration determined.