Human sPECAM-1 ELISA Kit

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  • Catalog
    E036311
  • species
    Human
  • GenesPECAM-1
  • Other Species Mouse sPECAM-1 ELISA KitMouse sPECAM-1/sCD31 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseHuman sPECAM-1 ELISA Kit allows for the in vitro quantitative determination of sPECAM-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilyCardiovascular; Immunology; Cytokines & Cell Signalling; Research Assays; Assay Categories
  • Product Description
    specifical
    Intended Use The human sPECAM-1 ELISA is an enzyme-linked immunosorbent assay for the detection of human sPECAM-1. For research use only, not for use in diagnostic procedures. PRINCIPLE OF THE TEST An anti-human sPECAM-1 coating antibody is adsorbed onto microwells. Human sPECAM-1 present in the unknown or calibrator binds to antibodies adsorbed to the microwells. A HRP-conjugated anti-human sPECAM-1 antibody is added and binds to human sPECAM-1 captured by the first antibody. Following incubation unbound HRP-conjugated anti-human sPECAM-1 is removed during a wash step, and substrate solution reactive with HRP is added to the wells. A coloured product is formed in proportion to the amount of human sPECAM-1 present in the unknown or calibrator. The reaction is terminated by addition of acid and absorbance is measured at 450 nm. A calibrator curve is prepared from 7 human sPECAM-1 calibrator dilutions and human sPECAM-1 concentration determined.


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