Human ACRV1 ELISA Kit

Alternative name
Human acrosomal vesicle protein 1 ELISA Kit;Human D11S4365 ELISA Kit;Human SP-10 ELISA Kit;Human SPACA2 ELISA Kit;Human acrosomal protein SP-10 ELISA Kit;Human sperm protein 10 ELISA Kit;
Catalog
E000363
species
Human
GeneACRV1
Standard Curve
Other Species Mouse Acrv1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of ACRV1. No significant cross-reactivity or interference between ACRV1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACRV1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman ACRV1 ELISA Kit allows for the in vitro quantitative determination of ACRV1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalIntended Uses: This ACRV1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACRV1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACRV1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ACRV1 antibody and an ACRV1-HRP conjugate. The assay sample and buffer are incubated together with ACRV1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACRV1 concentration since ACRV1 from samples and ACRV1-HRP conjugate compete for the anti-ACRV1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACRV1 from the sample, fewer sites are left to bind ACRV1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACRV1 concentration in each sample is interpolated from this standard curve.
Human Acrosomal protein SP-10 Protein information
Uniprot ID ASPX_HUMAN
Uniprot AC P26436; Q53FF4;
UniGene Hs.169222; Hs.596575;
GeneID 56
KEGG hsa:56;
Human Acrosomal protein SP-10 Protein SEQUENCE
SEQUENCE 265 AA; 28156 MW; 318216453F20D68A CRC64;
MNRFLLLMSL YLLGSARGTS SQPNELSGSI DHQTSVQQLP GEFFSLENPS
DAEALYETSS GLNTLSEHGS SEHGSSKHTV AEHTSGEHAE SEHASGEPAA
TEHAEGEHTV GEQPSGEQPS GEHLSGEQPL SELESGEQPS DEQPSGEHGS
GEQPSGEQAS GEQPSGEHAS GEQASGAPIS STSTGTILNC YTCAYMNDQG
KCLRGEGTCI TQNSQQCMLK KIFEGGKLQF MVQGCENMCP SMNLFSHGTR
MQIICCRNQS FCNKI
UCSC uc001qcl.4; human. [P26436-1];

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Human ACRV1 ELISA Kit

Human acrosomal vesicle protein 1 ELISA Kit;Human D11S4365 ELISA Kit;Human SP-10 ELISA Kit;Human SPACA2 ELISA Kit;Human acrosomal protein SP-10 ELISA Kit;Human sperm protein 10 ELISA Kit;