Human ACRV1 ELISA Kit

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  • Alternative name

    Human acrosomal vesicle protein 1 ELISA Kit;Human D11S4365 ELISA Kit;Human SP-10 ELISA Kit;Human SPACA2 ELISA Kit;Human acrosomal protein SP-10 ELISA Kit;Human sperm protein 10 ELISA Kit;

  • Catalog
    E000363
  • species
    Human
  • GeneACRV1
  • Standard CurveHuman ACRV1 ELISA Kit
  • Other Species Mouse Acrv1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ACRV1. No significant cross-reactivity or interference between ACRV1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ACRV1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman ACRV1 ELISA Kit allows for the in vitro quantitative determination of ACRV1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCancer
  • Product Description
    specifical
    Intended Uses: This ACRV1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ACRV1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ACRV1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ACRV1 antibody and an ACRV1-HRP conjugate. The assay sample and buffer are incubated together with ACRV1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ACRV1 concentration since ACRV1 from samples and ACRV1-HRP conjugate compete for the anti-ACRV1 antibody binding site. Since the number of sites is limited, as more sites are occupied by ACRV1 from the sample, fewer sites are left to bind ACRV1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ACRV1 concentration in each sample is interpolated from this standard curve.
  • Human Acrosomal protein SP-10 Protein information
  • Uniprot ID ASPX_HUMAN
  • Uniprot AC P26436; Q53FF4;
  • UniGene Hs.169222; Hs.596575;
  • GeneID 56
  • KEGG hsa:56;
  • Human Acrosomal protein SP-10 Protein SEQUENCE
  • SEQUENCE 265 AA; 28156 MW; 318216453F20D68A CRC64;

    MNRFLLLMSL YLLGSARGTS SQPNELSGSI DHQTSVQQLP GEFFSLENPS

    DAEALYETSS GLNTLSEHGS SEHGSSKHTV AEHTSGEHAE SEHASGEPAA

    TEHAEGEHTV GEQPSGEQPS GEHLSGEQPL SELESGEQPS DEQPSGEHGS

    GEQPSGEQAS GEQPSGEHAS GEQASGAPIS STSTGTILNC YTCAYMNDQG

    KCLRGEGTCI TQNSQQCMLK KIFEGGKLQF MVQGCENMCP SMNLFSHGTR

    MQIICCRNQS FCNKI

  • UCSC uc001qcl.4; human. [P26436-1];


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